Overview

  • Product name
    Anti-HPV18 E7 antibody [8E2]
  • Description
    Mouse monoclonal [8E2] to HPV18 E7
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human papillomavirus
  • Immunogen

    Recombinant full length HPV18 E7 protein

  • Epitope
    Mapped to amino acids 36-70
  • Positive control
    • WB: U20S, Hela and SiHa cell lysates. ICC/IF: U2OS cells transfected with plasmid expressing HPV18 E7 protein; Hela cells.
  • General notes
    Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Properties

Applications

Our Abpromise guarantee covers the use of ab100953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 12 kDa.
IP 1/500.
ELISA 1/128000.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
  • Database links
    • Alternative names
      • E7 protein antibody
      • Human Papilloma Virus Type 18 E7 protein antibody

    Images

    • All lanes : Anti-HPV18 E7 antibody [8E2] (ab100953) at 1/1000 dilution

      Lane 1 : lysate of U20S cells with plasmid expressing E7 protein
      Lane 2 : plain U20S lysate
      Lane 3 : Hela lysate. HeLa cells containing HPV-18 genome sequences expressing HPV E6 and E7 proteins.
      Lane 4 : SiHa lysate
      Lane 5 : Marker

      Predicted band size: 12 kDa

    • IP analysis of HPV18 E7 protein using ab100953 at 1/500:
      Lane 1: detection of E7 protein in lysate of U20S cells
      Lane 2: the lower band is precipitated E7, upper bands are light and heavy chain of antibody
      Lane 3: precipitation without ab100953
      Lane 4: IP exp. with plain U2OS cells
      Lane 5: IP exp. with non-transfected HeLa cells. Lower band corresponds to native HPV-18 E7 protein expressed by HPV-18 genome expressed in Hela cells.
      Lane 6: marker.

    • Immunofluorescent (IF) analysis of HPV18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E7 detected by ab100953. IF analysis of E7 protein shows similar result also in HeLa cells.

    References

    This product has been referenced in:
    • Morgan EL  et al. STAT3 activation by E6 is essential for the differentiation-dependent HPV18 life cycle. PLoS Pathog 14:e1006975 (2018). Read more (PubMed: 29630659) »
    • Wasson CW  et al. Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle. Oncotarget 8:103581-103600 (2017). Read more (PubMed: 29262586) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - other (human foreskin keratinocytes)
    Loading amount
    10 µg
    Specification
    human foreskin keratinocytes
    Gel Running Conditions
    Reduced Denaturing (15% Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up