Overview

  • Product name

  • Description

    Mouse polyclonal to HRASLS3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    HRASLS3 (AAH01387.1, 1 a.a. ~ 162 a.a) full-length human protein.

  • Positive control

    • Human placenta tissue lysate Transfected 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

Target

  • Function

    Exhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue (By similarity). N- and O-acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity.
  • Tissue specificity

    Widely expressed. low expression, if any, in hematopoietic cells and thymus. In testis, confined to round spermatids. Expressed in normal ovarian epithelial cells. Down-regulated in some ovarian carcinomas and testicular germ cell tumors.
  • Sequence similarities

    Belongs to the H-rev107 family.
  • Cellular localization

    Cytoplasm. Cytoplasm > perinuclear region. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adipose specific phospholipase A2 antibody
    • Adipose specific PLA2 antibody
    • Adipose-specific phospholipase A2 antibody
    • AdPLA antibody
    • Ca independent phospholipase A1/2 antibody
    • Group XVI phospholipase A1/A2 antibody
    • Group XVI phospholipase A2 antibody
    • H rev 107 protein homolog antibody
    • H REV107 1 antibody
    • H-rev 107 protein homolog antibody
    • HRAS like suppressor 1 antibody
    • HRAS like suppressor 3 antibody
    • HRAS-like suppressor 1 antibody
    • HRAS-like suppressor 3 antibody
    • HREV107 1 antibody
    • HREV107 3 antibody
    • HREV107 antibody
    • HREV107-1 antibody
    • HREV107-3 antibody
    • HRSL3 antibody
    • HRSL3_HUMAN antibody
    • Phospholipase A2 group XVI antibody
    • Pla2g16 antibody
    • Renal carcinoma antigen NY REN 65 antibody
    • Renal carcinoma antigen NY-REN-65 antibody
    see all

Images

  • Anti-HRASLS3 antibody (ab88447) at 1 µg/ml + Human placenta tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size: 18 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-HRASLS3 antibody (ab88447) at 1 µg/ml

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

References

ab88447 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your answer. I am happy to hear that this antibody is now producing good results for you. Please feel free to contact the Abcam Scientific Support Team with any further questions or concerns. We are happy to help. We just launched new products for Western Blotting. This includes precast gels that have a much longer shelf-life than conventional gels and and pre-stained molecular weight markers: https://www.abcam.com/index.html?pageconfig=resource&rid=14709 Click here (or use the following: https://www.abcam.com/index.html?datasheet=116028). I wish you good luck for your future research.

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