Key features and details
- Mouse polyclonal to HRASLS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HRASLS3 antibody
See all HRASLS3 primary antibodies
DescriptionMouse polyclonal to HRASLS3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
HRASLS3 (AAH01387.1, 1 a.a. ~ 162 a.a) full-length human protein.
- Human placenta tissue lysate Transfected 293T cell lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).|
FunctionExhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue (By similarity). N- and O-acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity.
Tissue specificityWidely expressed. low expression, if any, in hematopoietic cells and thymus. In testis, confined to round spermatids. Expressed in normal ovarian epithelial cells. Down-regulated in some ovarian carcinomas and testicular germ cell tumors.
Sequence similaritiesBelongs to the H-rev107 family.
Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Membrane.
- Information by UniProt
- Adipose specific phospholipase A2 antibody
- Adipose specific PLA2 antibody
- Adipose-specific phospholipase A2 antibody
Anti-HRASLS3 antibody (ab88447) at 1 µg/ml + Human placenta tissue lysate at 50 µg
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 18 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
All lanes : Anti-HRASLS3 antibody (ab88447) at 1 µg/ml
Lane 1 : Transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
ab88447 has not yet been referenced specifically in any publications.