Overview

  • Product name

  • Description

    Rabbit polyclonal to HRG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human, Pig
  • Immunogen

    Recombinant fragment corresponding to HRG aa 18-254. N-terminal His-tag and GST-tag. Expressed in E.coli.
    Sequence:

    MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDGSTSGSGHHHHHHSAGLVPRGSTAIGMKET AAAKFERQHMDSPDLGTLEVLFQGPLGSEFAVSPTDCSAVEPEAEKALDL INKRRRDGYLFQLLRIADAHLDRVENTTVYYLVLDVQESDCSVLSRKYWN DCEPPDSRRPSEIVIGQCKVIATRHSHESQDLRVIDFNCTTSSVSSALAN TKDSPVLIDFFEDTERYRKQANKALEKYKEENDDFASFRVDRIERVARVR GGEGTGYFVDFSVRNCPRHHFPRHPNVFGFCRADLFYDVEALDLESPKNL VINCEVFDPQEHENING


    Database link: P04196

  • Positive control

    • WB: Recombinant human HRG protein; K562 cell lysate; Pig liver lysate. IHC-P: Human liver tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 60 kDa.

Target

  • Relevance

    HRG (histidine-rich glycoprotein) contains two cystatin-like domains and is located in plasma and platelets. The physiological function is not yet known. It binds heme, dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin, thrombospondin, and the lysine-binding site of plasminogen. A potential prothrombotic effect of HRG is indicated by the inhibition of fibrinolysis and the reduction of inhibition of coagulation. Mutations in HRG lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • DKFZp779H1622 antibody
    • Histidine proline rich glycoprotein antibody
    • Histidine rich glycoprotein antibody
    • HPRG antibody
    • HRGP antibody
    • Thrombophilia due to elevated HRG antibody
    see all

Images

  • Anti-HRG antibody (ab231336) at 2 µg/ml + Recombinant human HRG protein.

    Predicted band size: 60 kDa

  • Anti-HRG antibody (ab231336) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate.

    Predicted band size: 60 kDa

  • Anti-HRG antibody (ab231336) at 2 µg/ml + Pig liver tissue lysate.

    Predicted band size: 60 kDa

  • Formalin-fixed, paraffin-embedded human liver tissue stained for HRG using ab231336 at 20 mg/ml in immunohistochemical analysis. DAB staining.

References

ab231336 has not yet been referenced specifically in any publications.

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