Overview

  • Product name

    Anti-HRG beta 1 antibody
  • Description

    Rabbit polyclonal to HRG beta 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Sandwich ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant Human HRG beta 1.

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

  • Form

    Lyophilised:Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human HRG beta 1. Can be used as capture antibody when paired with ab87549.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 70 kDa.

Target

  • Relevance

    HRG beta 1 is an isoform from the Neuregulin/Heregulin family of structurally related polypeptide growth factors which are derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. HRG1-ß1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. Although HRG1-ß1 biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility promoting factor (AMF).
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Acetylcholine receptor inducing activity antibody
    • ARIA antibody
    • Breast cancer cell differentiation factor p45 antibody
    • GGF antibody
    • GGF2 antibody
    • glial growth factor antibody
    • Heregulin antibody
    • Heregulin beta1 antibody
    • heregulin, alpha (45kD, ERBB2 p185 activator) antibody
    • HGL antibody
    • HRG antibody
    • HRG beta 1A antibody
    • HRG1 antibody
    • HRGA antibody
    • NDF antibody
    • neu differentiation factor antibody
    • Neuregulin 1 antibody
    • NRG1 antibody
    • sensory and motor neuron derived factor antibody
    • SMDF antibody
    see all

Images

  • IHC image of HRG beta 1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87547, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab87547 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

I compared the homology of the sequence and Pro-neuregulin-1, membrane-bound isoforms (Q02297 NRG1_HUMAN). The only isoforms which had 100% were 6 (beta1, 71kDa) and 11 (Type IV-beta1a, 65,5kDa). 100%-isoforms 6,11 84%-isoforms 7 83%-isoforms 8,9,10 80%-isoforms 2 64%- isoforms 1,3,4 Therefore the antibody is specific for beta 1 in addition to Type IV-beta1a. We have not carried out any neutralising experiments.

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