The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 56 kDa.
Use at an assay dependent concentration.
Use a concentration of 2 - 4 µg/ml.
In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.
Belongs to the G-protein coupled receptor 1 family.
Potential sites of phosphorylation in the third cytoplasmic loop may play an important role in regulating signal transduction through the receptor molecule.
Immunohistochemistry (paraffin-embedded sections) analysis of human lung tissue labeling HRH1 with ab77100 at 4µg/ml. Microwaved antigen retrieval was performed using Tris/EDTA buffer pH 6. HRP staining.
Western blot - Anti-HRH1 antibody (ab77100)
Anti-HRH1 antibody (ab77100) at 0.5 µg/ml + Heart lysate (in RIPA buffer) at 35 µg
Predicted band size: 56 kDa Observed band size: 56 kDa