Anti-HRH1/H1R antibody (ab75236)
Key features and details
- Rabbit polyclonal to HRH1/H1R
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HRH1/H1R antibody
See all HRH1/H1R primary antibodies -
Description
Rabbit polyclonal to HRH1/H1R -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human HRH1/H1R (internal sequence).
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Positive control
- COLO205 cell extracts. LOVO cells.
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General notes
Previously labelled as HRH1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab75236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). | |
| ICC/IF | 1/500 - 1/1000. |
Target
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Function
In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPotential sites of phosphorylation in the third cytoplasmic loop may play an important role in regulating signal transduction through the receptor molecule. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3269 Human
- Omim: 600167 Human
- SwissProt: P35367 Human
- Unigene: 1570 Human
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Alternative names
- BPHS, mouse, homolog of antibody
- H1 R antibody
- H1R antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HRH1/H1R antibody (ab75236)
ab75236 at 1/500 dilution staining HRH1/H1R in LOVO cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Western blot - Anti-HRH1/H1R antibody (ab75236)All lanes : Anti-HRH1/H1R antibody (ab75236) at 1/500 dilution
Lane 1 : COLO205 cell extracts
Lane 2 : COLO205 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Protocols
References (0)
ab75236 has not yet been referenced specifically in any publications.
