Overview

  • Product name
  • Description
    Goat polyclonal to HRH3 / H3R
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human HRH3/ H3R aa 135-149 (internal sequence) (Cysteine residue).
    Sequence:

    SVTRAVSYRAQQGDT

  • Positive control
    • A549 cell line lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.05% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156810 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).

Target

  • Function
    The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization.
  • Tissue specificity
    Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 97 antibody
    • G-protein coupled receptor 97 antibody
    • GPCR 97 antibody
    • GPCR97 antibody
    • H3R antibody
    • HH 3R antibody
    • HH3 R antibody
    • HH3R antibody
    • Histamine H3 receptor antibody
    • Histamine receptor H3 antibody
    • HRH 3 antibody
    • HRH3 antibody
    • HRH3_HUMAN antibody
    • OTTHUMP00000031459 antibody
    see all

Images

  • Anti-HRH3 / H3R antibody (ab156810) at 0.1 µg/ml + A549 cell line lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 49 kDa
    Observed band size: 48 kDa (why is the actual band size different from the predicted?)

References

ab156810 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab156810.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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