Overview

  • Product name

  • Description

    Rabbit polyclonal to HRH3/H3R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human HRH3/H3R aa 236-332.
    Database link: Q9Y5N1

  • Positive control

    • WB: A549 whole cell lysate. IHC-P: Human brain tissue. ICC/IF: A549 cells.
  • General notes

    Previously labelled as HRH3. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 49 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.

Target

  • Function

    The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization.
  • Tissue specificity

    Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • G protein coupled receptor 97 antibody
    • G-protein coupled receptor 97 antibody
    • GPCR 97 antibody
    • GPCR97 antibody
    • H3R antibody
    • HH 3R antibody
    • HH3 R antibody
    • HH3R antibody
    • Histamine H3 receptor antibody
    • Histamine receptor H3 antibody
    • HRH 3 antibody
    • HRH3 antibody
    • HRH3_HUMAN antibody
    • OTTHUMP00000031459 antibody
    see all

Images

  • Paraffin-embedded human brain tissue stained for HRH3/H3R using ab236952 at 1/100 dilution in immunohistochemical analysis.

  • Anti-HRH3/H3R antibody (ab236952) at 1/500 dilution + A549 (human lung carcinoma cell line) cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 49 kDa

  • A549 (human lung carcinoma cell line) cells stained for HRH3/HR3 (green) using ab236952 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).

References

ab236952 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236952.
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