Anti-HRH3/H3R antibody (ab96787)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-HRH3/H3R antibody
See all HRH3/H3R primary antibodies -
Description
Rabbit polyclonal to HRH3/H3R -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey
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Immunogen
Synthetic peptide corresponding to Human HRH3/H3R aa 293-357.
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Positive control
- A431, H1299, HeLa and HepG2 cell lysates
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General notes
Previously labelled as HRH3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
Target
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Function
The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization. -
Tissue specificity
Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 11255 Human
- Omim: 604525 Human
- SwissProt: Q9Y5N1 Human
- Unigene: 251399 Human
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Alternative names
- G protein coupled receptor 97 antibody
- G-protein coupled receptor 97 antibody
- GPCR 97 antibody
see all
Images
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Western blot - Anti-HRH3/H3R antibody (ab96787)All lanes : Anti-HRH3/H3R antibody (ab96787) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS PAGE
Protocols
References
ab96787 has not yet been referenced specifically in any publications.
