Overview

  • Product name

    Anti-HRH3/H3R antibody - Cytoplasmic domain
    See all HRH3/H3R primary antibodies
  • Description

    Rabbit polyclonal to HRH3/H3R - Cytoplasmic domain
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Hamster, Cow, Dog, Monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide corresponding to Human HRH3/H3R. 16 amino acid peptide from 2nd cytoplasmic domain. NP_009163.2.
    Database link: Q9Y5N1

  • Positive control

    • Human brain (neurons and glia) tissue.
  • General notes

    Previously labelled as HRH3. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 7 - 15 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization.
    • Tissue specificity

      Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • G protein coupled receptor 97 antibody
      • G-protein coupled receptor 97 antibody
      • GPCR 97 antibody
      • GPCR97 antibody
      • H3R antibody
      • HH 3R antibody
      • HH3 R antibody
      • HH3R antibody
      • Histamine H3 receptor antibody
      • Histamine receptor H3 antibody
      • HRH 3 antibody
      • HRH3 antibody
      • HRH3_HUMAN antibody
      • OTTHUMP00000031459 antibody
      see all

    Images

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain (neurons and glia) tissue, labeling HRH3/H3R with ab188972 at 15 µg/ml.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain (neurons) tissue, labeling HRH3/H3R with ab188972 at 15 µg/ml.

    References

    ab188972 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab188972.
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