Overview

  • Product name

    Anti-HRH3/H3R antibody [EPR5631]
    See all HRH3/H3R primary antibodies
  • Description

    Rabbit monoclonal [EPR5631] to HRH3/H3R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HRH3/H3R (N terminal). The exact sequence is proprietary. (In the extracellular domain).
    Database link: Q9Y5N1

  • Positive control

    • Lysates of: Jurkat, SH-SY5Y, C6, A549, Neuro-2a Jurkat cells
  • General notes

    Previously labelled as HRH3. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.62 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5631
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization.
    • Tissue specificity

      Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • G protein coupled receptor 97 antibody
      • G-protein coupled receptor 97 antibody
      • GPCR 97 antibody
      • GPCR97 antibody
      • H3R antibody
      • HH 3R antibody
      • HH3 R antibody
      • HH3R antibody
      • Histamine H3 receptor antibody
      • Histamine receptor H3 antibody
      • HRH 3 antibody
      • HRH3 antibody
      • HRH3_HUMAN antibody
      • OTTHUMP00000031459 antibody
      see all

    Images

    • All lanes : Anti-HRH3/H3R antibody [EPR5631] (ab124732)

      Lane 1 : Jurkat cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 49 kDa

    • Flow cytometric analysis of Jurkat cells using ab124732 (red)at 1/100 dilution or a rabbit IgG (negative) (green)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Chen J & Hu XY Inhibition of histamine receptor H3R suppresses prostate cancer growth, invasion and increases apoptosis via the AR pathway. Oncol Lett 16:4921-4928 (2018). Read more (PubMed: 30250558) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Vielen Dank für Ihre Email. Ich werde Ihnen gerne einen unserer anderen HRH3 Antikörper als Austauschprodukt zukommen lassen.

    Wir bieten 5 andere HRH3 Antikörper an. Ich habe Ihnen eine Liste gemacht und es wäre hilfreich wenn Sie sich den Antikörper davon aussuchen, der am besten Ihren Anforderungen entspricht.

    1. https://www.abcam.com/HRH3-antibody-ab56935.html

    2. https://www.abcam.com/HRH3-antibody-ab84468.html

    3. https://www.abcam.com/HRH3-antibody-ab96787.html

    4. https://www.abcam.com/HRH3-antibody-ab97450.html

    5. https://www.abcam.com/HRH3-antibody-C-terminal-ab135500.html

    Sobald Sie mich wissen lassen welches dieser Produkte für Sie in Frage kommt werde ich veranlassen, dass ein kostenloses Ersatzprodukt an Sie gesendet wird. Wenn Sie weitere Fragen haben zögern Sie bitte nicht sich an mich zu wenden.

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    Answer

    Sie haben Recht, dass Ihre Ergebnisse Besorgnis erregend sind, bezüglich der Spezifität des Antikörpers. Das Labor ist momentan dabei diesen Antikörper erneut auf seine Spezifität zu testen und wird sich bei mir melden sobald neue Ergebnisse vorliegen. Ich werde diese dann gerne an Sie weiter leiten.

    Ich möchte Ihnen hiermit noch einmal für Ihre Rückmeldung danken. Wir nehmen die Qualität unserer Antikörper sehr ernst und sind dafür auf Kundenrueckmeldungen wie die Ihre angewiesen.

    Bitte lassen Sie mich wissen wie Sie in der Zwischenzeit verfahren wollen.

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    Question
    Answer

    Thank you for contacting us. Please find the requested datasheet attached. Let me know if you need any additional information or assistance.

    Read More

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