Anti-Hrk antibody (ab45419)
Key features and details
- Rabbit polyclonal to Hrk
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Hrk antibody -
Description
Rabbit polyclonal to Hrk -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide of 15 amino acids in length from near the center of human Hrk
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Positive control
- Mouse pancreas tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 10 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 10 kDa). |
Target
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Function
Promotes apoptosis. -
Domain
The BH3 motif is required for the induction of cell death. -
Cellular localization
Membrane. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 8739 Human
- Entrez Gene: 12123 Mouse
- Omim: 603447 Human
- SwissProt: O00198 Human
- SwissProt: P62816 Mouse
- Unigene: 87247 Human
- Unigene: 384468 Mouse
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Alternative names
- Activator of apoptosis harakiri antibody
- Activator of apoptosis Hrk antibody
- BCL2 interacting protein antibody
see all
Images
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Lane 1 : Anti-Hrk antibody (ab45419) at 2.5 µg/ml
Lane 2 : Anti-Hrk antibody (ab45419) at 5 µg/ml
All lanes : Mouse pancreas tissue lysate
Predicted band size: 10 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab45419 has been referenced in 5 publications.
- Dunn S et al. AKT-mTORC1 reactivation is the dominant resistance driver for PI3Kβ/AKT inhibitors in PTEN-null breast cancer and can be overcome by combining with Mcl-1 inhibitors. Oncogene 41:5046-5060 (2022). PubMed: 36241868
- Liang R et al. The Stress-Inducible BCL2A1 Is Required for Ovarian Cancer Metastatic Progression in the Peritoneal Microenvironment. Cancers (Basel) 13:N/A (2021). PubMed: 34572804
- Duval N et al. The Bcl-2 Homology-3 Domain (BH3)-Only Proteins, Bid, DP5/Hrk, and BNip3L, Are Upregulated in Reactive Astrocytes of End-Stage Mutant SOD1 Mouse Spinal Cord. Front Cell Neurosci 12:15 (2018). WB ; Mouse . PubMed: 29440992
- Li D et al. Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4. Nat Commun 9:2829 (2018). PubMed: 30026490
- Wang NN et al. Molecular targeting of the oncoprotein PLK1 in pediatric acute myeloid leukemia: RO3280, a novel PLK1 inhibitor, induces apoptosis in leukemia cells. Int J Mol Sci 16:1266-92 (2015). PubMed: 25574601