HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491)
![Western blot - HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491)](https://www.abcam.com/ps/products/198/ab198491/Images/ab198491-257504-WBab1984911.jpg "Western blot - HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491)")
Key features and details
- HRP Mouse monoclonal [7A12AF6] to 3-Nitrotyrosine
- Suitable for: WB
- Reacts with: Cow, Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-3-Nitrotyrosine antibody [7A12AF6]
See all 3-Nitrotyrosine primary antibodies -
Description
HRP Mouse monoclonal [7A12AF6] to 3-Nitrotyrosine -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Mouse, Rat
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Immunogen
Chemical/ Small Molecule corresponding to 3-Nitrotyrosine. Nitrated KLH (Keyhole Limpet Hemocyanin).
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Positive control
- WB: Nitrated liver homogenate and nitrated bovine serum albumin.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
7A12AF6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab198491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. |
Target
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Relevance
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis. -
Alternative names
- Nitrotyrosine antibody
Images
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Western blot - HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491)All lanes : HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491) at 1/5000 dilution
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Nitrated Human Liver Homogenate Standard (ab131380)
Lane 3 : Bovine Serum Albumin
Lane 4 :Bovine Serum Albumin (nitrated ) (ab131379)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 secondThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198491 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
References (0)
ab198491 has not yet been referenced specifically in any publications.
