Key features and details
- HRP Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
- Suitable for: WB
- Reacts with: Rat
- Conjugation: HRP
- Isotype: IgG1
Product nameHRP Anti-58K Golgi protein antibody [58K-9] - Golgi Marker
See all 58K Golgi protein primary antibodies
DescriptionHRP Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Hamster, Cow, Dog, Human, Pig, Monkey, African green monkey
- WB: Rat Liver tissue lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab202001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
FunctionFolate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi.
PathwayAmino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion.
Involvement in diseaseDefects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
Sequence similaritiesIn the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family.
Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
- Information by UniProt
- Formimidoyltetrahydrofolate cyclodeaminase antibody
- Formimidoyltransferase cyclodeaminase antibody
- Formiminotetrahydrofolate cyclodeaminase antibody
HRP Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab202001) at 1/5000 dilution + Liver (Rat) Tissue Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202001 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab202001 has not yet been referenced specifically in any publications.