HRP Anti-6X His tag® antibody [4D11] (ab202004)
![Western blot - HRP Anti-6X His tag® antibody [4D11] (ab202004)](https://www.abcam.com/ps/products/202/ab202004/Images/ab202004-246443-anti-6x-his-tag-antibody-4d11-hrp-western-blot.jpg "Western blot - HRP Anti-6X His tag® antibody [4D11] (ab202004)")
Key features and details
- HRP Mouse monoclonal [4D11] to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-6X His tag® antibody [4D11]
See all 6X His tag® primary antibodies -
Description
HRP Mouse monoclonal [4D11] to 6X His tag® -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Epitope
This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50). -
Positive control
- WB: E. coli Positive Control (Escherichia coli ) Whole Cell Lysate
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
4D11 -
Myeloma
NS1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab202004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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Western blot - HRP Anti-6X His tag® antibody [4D11] (ab202004)HRP Anti-6X His tag® antibody [4D11] (ab202004) at 1/5000 dilution +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202004 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
References (0)
ab202004 has not yet been referenced specifically in any publications.
