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  1. Link

    hrp-6x-his-tag-antibody-ab1187.pdf

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Tags & Cell Markers Epitope Tags His Tag
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HRP Anti-6X His tag® antibody (ab1187)

  • Datasheet
  • SDS
Reviews (12)Q&A (5)References (98)

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Western blot - HRP Anti-6X His tag® antibody (ab1187)

    Key features and details

    • HRP Rabbit polyclonal to 6X His tag®
    • Suitable for: ELISA, WB
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG

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    View more associated products

    Overview

    • Product name

      HRP Anti-6X His tag® antibody
      See all 6X His tag® primary antibodies
    • Description

      HRP Rabbit polyclonal to 6X His tag®
    • Host species

      Rabbit
    • Conjugation

      HRP
    • Tested applications

      Suitable for: ELISA, WBmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) can be used as a positive control in WB.
    • General notes

      Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

      See other anti-rabbit secondary antibodies that can be used with this antibody.

      HIS-TAG® is a trademark of EMD Biosciences, Inc.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.00
      Preservative: 0.002% Thimerosal (merthiolate)
      Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • His Tag

    Associated products

    • Positive Controls

      • E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431)
    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)
      • HRP Anti-6X His tag® antibody [4D11] (ab202004)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1187 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA (3)
    1/5000 - 1/20000.
    WB (9)
    1/1000 - 1/5000.
    Notes
    ELISA
    1/5000 - 1/20000.
    WB
    1/1000 - 1/5000.

    Target

    • Relevance

      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells.
    • Cellular localization

      Depends upon the localization of the parent protein tagged with hexahistidine.
    • Alternative names

      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    Images

    • Western blot - HRP Anti-6X His tag® antibody (ab1187)
      Western blot - HRP Anti-6X His tag® antibody (ab1187)This image is courtesy of an Abreview submitted by Mr Ohrt Thomas
      All lanes : HRP Anti-6X His tag® antibody (ab1187) at 1/4000 dilution

      Lane 1 : 4ug Human His-tagged protein purified from E.coli
      Lane 2 : 2ug Human His-tagged protein purified from E.coli
      Lane 3 : 1ug Human His-tagged protein purified from E.coli
      Lane 4 : 0.5ug Human His-tagged protein purified from E.coli

      Observed band size: 136 kDa why is the actual band size different from the predicted?

      See Abreview

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (98)

    Publishing research using ab1187? Please let us know so that we can cite the reference in this datasheet.

    ab1187 has been referenced in 98 publications.

    • Madrid R  et al. Survey of Commercial Food Products for Detection of Walnut (Juglans regia) by Two ELISA Methods and Real Time PCR. Foods 10:N/A (2021). PubMed: 33671390
    • Cho G  et al. Structural insights into phosphatidylethanolamine formation in bacterial membrane biogenesis. Sci Rep 11:5785 (2021). PubMed: 33707636
    • Martínez-Hernández SL  et al. Evaluation of the PE?III-LC3-KDEL3 Chimeric Protein of Entamoeba histolytica-Lectin as a Vaccine Candidate against Amebic Liver Abscess. J Immunol Res 2021:6697900 (2021). PubMed: 33824880
    • Kotoucek J  et al. Characterization and purification of pentameric chimeric protein particles using asymmetric flow field-flow fractionation coupled with multiple detectors. Anal Bioanal Chem 413:3749-3761 (2021). PubMed: 33837800
    • Hershewe JM  et al. Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles. Nat Commun 12:2363 (2021). PubMed: 33888690
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 17 Abreviews or Q&A

    Western blot abreview for Anti-6X His tag® antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse pancreatic cancer primary cell lines)
    Loading amount
    25 µg
    Specification
    mouse pancreatic cancer primary cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 09 2011

    Western blot abreview for Anti-6X His tag® antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Recombinant protein (Overexpressed his-tagged protein in a eColi cell c)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    1 µg
    Specification
    Overexpressed his-tagged protein in a eColi cell c
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 13 2020

    Western blot

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    0.1 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Recombinant protein (purification sur colonne HIS Trap)
    Specification
    purification sur colonne HIS Trap
    Blocking step
    caséine as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 30 2013

    Western blot abreview for Anti-6X His tag® antibody (HRP)

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Purified protein (purified His-tagged lipase, SPINK1)
    Loading amount
    1 µg
    Specification
    purified His-tagged lipase, SPINK1
    Gel Running Conditions
    Reduced Denaturing (15%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Miklos Sahin-Toth

    Verified customer

    Submitted Aug 12 2010

    Western blot abreview for Anti-6X His tag® antibody (HRP)

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (neuronal cell)
    Loading amount
    20 µg
    Specification
    neuronal cell
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 09 2010

    Western blot abreview for Anti-6X His tag® antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (Purified His-tagged extracellular human LDLR)
    Loading amount
    0.1 µg
    Specification
    Purified His-tagged extracellular human LDLR
    Gel Running Conditions
    Reduced Denaturing (4-20% gel)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 25 2009

    ELISA abreview for Anti-6X His tag® antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Escherichia coli Purified protein (BL21(DE3))
    Specification
    BL21(DE3)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Type
    Sandwich (Capture)
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 27 2009

    Western blot abreview for Anti-6X His tag® antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Purified protein (BL21(DE3))
    Loading amount
    0.01 µg
    Specification
    BL21(DE3)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 30 2008

    Western blot abreview for Anti-6X His tag® antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (recombinant human fibronectin domain expressed in)
    Loading amount
    1 µg
    Specification
    recombinant human fibronectin domain expressed in
    Gel Running Conditions
    Reduced Denaturing (12% BisTris NuPAGE gel)
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 09 2008

    Question

    Can this antibody be stored at -20C?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 01 2011

    Answer

     Thank you for calling Abcam earlier. After having spoken to the lab, I would advise you not to store ab1187 at at -20C as it will cause the product to lose some functionality due to the HRP conjugation.

    Read More

    Abcam Scientific Support

    Answered on Dec 01 2011

    1-10 of 17 Abreviews or Q&A

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