HRP Anti-6X His tag® antibody (ab3553)
Key features and details
- HRP Chicken polyclonal to 6X His tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgY
Overview
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Product name
HRP Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
HRP Chicken polyclonal to 6X His tag® -
Host species
Chicken -
Conjugation
HRP -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
-
General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Myeloma
unknown -
Isotype
IgY -
Light chain type
unknown -
Research areas
Associated products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. 1/1000.
|
| ELISA |
Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. 1/1000. |
|
ELISA
Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification. |
Target
-
Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab3553 has been referenced in 7 publications.
- Chan CH et al. MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake. Int J Mol Sci 22:N/A (2021). PubMed: 33498219
- Manning JA et al. Dietary sodium modulates nephropathy in Nedd4-2-deficient mice. Cell Death Differ 27:1832-1843 (2020). PubMed: 31802037
- Burnside D et al. In Silico Engineering of Synthetic Binding Proteins from Random Amino Acid Sequences. iScience 11:375-387 (2019). PubMed: 30660105
- Miao J et al. Wnt/ß-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction. Aging Cell 18:e13004 (2019). PubMed: 31318148
- Dragovic SM et al. Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice. Cell Host Microbe 23:523-535.e5 (2018). PubMed: 29649443
- Henshall TL et al. Deletion of Nedd4-2 results in progressive kidney disease in mice. Cell Death Differ 24:2150-2160 (2017). IHC-P ; Mouse . PubMed: 28862701
- Allan CM et al. A conserved START domain coenzyme Q-binding polypeptide is required for efficient Q biosynthesis, respiratory electron transport, and antioxidant function in Saccharomyces cerevisiae. Biochim Biophys Acta 1831:776-791 (2013). PubMed: 23270816