HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)

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Western blot - HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)

    Key features and details

    • HRP Mouse monoclonal [AD1.1.10] to 6X His tag®
    • Suitable for: WB, ELISA
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG

    Overview

    • Product name

      HRP Anti-6X His tag® antibody [AD1.1.10]
      See all 6X His tag® primary antibodies

    • Description

      HRP Mouse monoclonal [AD1.1.10] to 6X His tag®

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WB, ELISAmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Other Immunogen Type corresponding to 6X His tag®. Antibody was raised against a polypeptide containing a tag

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Lyophilized:Reconstititue to 1.0 ml with distilled deionized water vortex gently and allow vial to sit on ice for 20 minutes; concentration will be 0.1mg/ml. We recommend that the investigator determine the appropriate working concentration for their specific application. Avoid exposure to heat and light.

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.

    • Storage buffer

      pH: 7.40
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.4% Sodium phosphate, 0.58% Sodium chloride, 1.5% BSA, 1.71% Sucrose

      Buffer after reconstitution in 1.0 ml distilled deionized water.
    • Concentration information loading...

    • Purity

      Size exclusion

    • Clonality

      Monoclonal

    • Clone number

      AD1.1.10

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab178563 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration.
    ELISA Use at an assay dependent concentration.

    Target

    • Relevance

      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

    • Cellular localization

      Depends upon the localization of the parent protein tagged with hexahistidine.

    • Alternative names

      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    Images

    • Western blot - HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
      Western blot - HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)

      Western Blot of a 41kDa protein fused (expressed in E.Coli) to His tag using ab178563 0.25 μg/ml. Loading was confirmed by Coomassi blue staining of transferred proteins. 

    References (1)

    Publishing research using ab178563? Please let us know so that we can cite the reference in this datasheet.

    ab178563 has been referenced in 1 publication.

    • Khoshtinat Nikkhoi S  et al. Multivalent targeting and killing of HER2 overexpressing breast carcinoma cells with methotrexate-encapsulated tetra-specific non-overlapping variable domain heavy chain anti-HER2 antibody-PEG-liposomes: In vitro proof-of-concept. Eur J Pharm Sci 122:42-50 (2018). PubMed: 29933078

    Customer reviews and Q&As

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    Question

    How was this antibody tested for western blotting? In particular, what dilutions were tested and which gave the optimal result?

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    Answer

    For staining western blots with this conjugate, we recommend a starting concentration of 0.2-0.5 ug/ml (1:500- 1:200 of a 0.1 mg/ml stock) for detection of his-tagged proteins on a western blot. However, as with every conjugate product such as this, we also recommend determining the best working concentration by experimental titration to account for differences in buffers, wash procedures, substrates, etc.

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