Recombinant HRP Anti-6X His tag® antibody [EPR20547] (ab237339)
![Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339)](https://www.abcam.com/ps/products/237/ab237339/Images/ab237339-387589-anti-6x-his-tag-antibody-epr20547-hrp-western-blot-histone-h3-recombinat-ab198757-4-minute-exposure-empty-histone-h4-recombinant-ab198115.jpg "Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR20547] to 6X His tag®
- Suitable for: WB
- Reacts with: Recombinant fragment
- Conjugation: HRP
Overview
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Product name
HRP Anti-6X His tag® antibody [EPR20547]
See all 6X His tag® primary antibodies -
Description
HRP Rabbit monoclonal [EPR20547] to 6X His tag® -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide within 6X His tag®. The exact sequence is proprietary.
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Positive control
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, PBS, 30% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20547 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
- Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (ab232492)
- Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (ab237336)
- Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337)
- PE Anti-6X His tag® antibody [EPR20547] (ab237338)
Applications
Our Abpromise guarantee covers the use of ab237339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339)All lanes : HRP Anti-6X His tag® antibody [EPR20547] (ab237339) at 1/5000 dilution
Lane 1 : Histone H3 recombinant (ab198757) (4 minute exposure) cell lysate
Lane 2 : Histone H4 recombinant (ab198115) (1 minute 30 seconds exposure) cell lysate
Lane 3 : Histone H2A recombinant (ab200295)(1 minute 30 seconds exposure) cell lysate
Lysates/proteins at 2 µg per lane.
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?ab237339 was shown to react with 6X His tag® in western blot. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab237339 overnight at 4°C at a 1 in 5000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.
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![HRP Anti-6X His tag® antibody [EPR20547] (ab237339)](https://www.abcam.com/ps/products/237/ab237339/Images/ab237339-2-benefits-of-recombinant-antibodies.png)
HRP Anti-6X His tag® antibody [EPR20547] (ab237339)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab237339 has not yet been referenced specifically in any publications.
