For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    hrp-abce1-antibody-epr15373b-c-terminal-ab209043.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Adapters Transmembrane
Share by email
RecombinantRabMAb

Recombinant HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)
  • HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal
    See all ABCE1 primary antibodies
  • Description

    HRP Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ABCE1 aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: P61221

  • Positive control

    • WB: HEK293, K562, HeLa cell lysates
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15373(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Transmembrane
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling

Associated products

  • Alternative Versions

    • Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    • PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab212001)
    • Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • K562 whole cell lysate (ab7911)

Applications

Our Abpromise guarantee covers the use of ab209043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

Target

  • Function

    Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCE family.
    Contains 2 4Fe-4S ferredoxin-type domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
  • Target information above from: UniProt accession P61221 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6059 Human
    • Entrez Gene: 24015 Mouse
    • Entrez Gene: 361390 Rat
    • Omim: 601213 Human
    • SwissProt: P61221 Human
    • SwissProt: P61222 Mouse
    • Unigene: 12013 Human
    • Unigene: 5831 Mouse
    • Alternative names

      • 2' 5' oligoadenylate binding protein antibody
      • 2''-5''-oligoadenylate-binding protein antibody
      • 2'5' oligoadenylate binding protein antibody
      • ABC 38 antibody
      • ABC38 antibody
      • ABCE 1 antibody
      • ABCE1 antibody
      • ABCE1_HUMAN antibody
      • ATP binding cassette sub family E (OABP) member 1 antibody
      • ATP binding cassette sub family E member 1 antibody
      • ATP-binding cassette sub-family E member 1 antibody
      • HuHP 68 antibody
      • HuHP68 antibody
      • OABP antibody
      • Ribonuclease 4 inhibitor antibody
      • Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) inhibitor antibody
      • Ribonuclease L (2'5' oligoisoadenylate synthetase dependent) inhibitor antibody
      • Ribonuclease L inhibitor antibody
      • RLI antibody
      • RNase L inhibitor antibody
      • RNASEL1 antibody
      • RNASELI antibody
      • RNS 4I antibody
      • RNS4I antibody
      see all

    Images

    • Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)
      Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)
      All lanes : HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043) at 1/5000 dilution

      Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 67 kDa
      Observed band size: 67 kDa


      Exposure time: 2 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209043 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)
      HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab209043? Please let us know so that we can cite the reference in this datasheet.

    ab209043 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab209043.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.