Recombinant HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal
See all ABCE1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ABCE1 aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: P61221 -
Positive control
- WB: HEK293, K562, HeLa cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15373(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab209043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/5000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCE family.
Contains 2 4Fe-4S ferredoxin-type domains.
Contains 2 ABC transporter domains. -
Cellular localization
Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 6059 Human
- Entrez Gene: 24015 Mouse
- Entrez Gene: 361390 Rat
- Omim: 601213 Human
- SwissProt: P61221 Human
- SwissProt: P61222 Mouse
- Unigene: 12013 Human
- Unigene: 5831 Mouse
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Alternative names
- 2' 5' oligoadenylate binding protein antibody
- 2''-5''-oligoadenylate-binding protein antibody
- 2'5' oligoadenylate binding protein antibody
see all
Images
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All lanes : HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043) at 1/5000 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209043 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
References (0)
ab209043 has not yet been referenced specifically in any publications.