Recombinant HRP Anti-ABCG1 antibody [EP1366Y] (ab201776)
![Western blot - HRP Anti-ABCG1 antibody [EP1366Y] (ab201776)](https://www.abcam.com/ps/products/201/ab201776/Images/ab201776-247936-WBab2017761.jpg "Western blot - HRP Anti-ABCG1 antibody [EP1366Y] (ab201776)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1366Y] to ABCG1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-ABCG1 antibody [EP1366Y]
See all ABCG1 primary antibodies -
Description
HRP Rabbit monoclonal [EP1366Y] to ABCG1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide within Human ABCG1 aa 150-250. The exact sequence is proprietary.
Database link: P45844 -
Positive control
- WB: HUVEC whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1366Y -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab201776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa). |
Target
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Function
Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. -
Tissue specificity
Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes. - Information by UniProt
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Database links
- Entrez Gene: 510745 Cow
- Entrez Gene: 9619 Human
- Entrez Gene: 85264 Rat
- Omim: 603076 Human
- SwissProt: P45844 Human
- Unigene: 124649 Human
- Unigene: 8398 Rat
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Alternative names
- ABC transporter 8 antibody
- ABC8 antibody
- ABCG1 antibody
see all
Images
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Western blot - HRP Anti-ABCG1 antibody [EP1366Y] (ab201776)HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg with
HRP Anti-ABCG1 antibody [EP1366Y] (ab201776) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201776 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-ABCG1 antibody [EP1366Y] (ab201776)](https://www.abcam.com/ps/products/201/ab201776/Images/ab201776-3-benefits-of-recombinant-antibodies.png)
HRP Anti-ABCG1 antibody [EP1366Y] (ab201776)
References (1)
ab201776 has been referenced in 1 publication.
- Hang L et al. Ox-LDL Causes Endothelial Cell Injury Through ASK1/NLRP3-Mediated Inflammasome Activation via Endoplasmic Reticulum Stress. Drug Des Devel Ther 14:731-744 (2020). PubMed: 32158192
