HRP Anti-ADA antibody (ab34651)

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Key features and details

  • HRP Rabbit polyclonal to ADA
  • Reacts with: Cow
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-ADA antibody
    See all ADA primary antibodies

  • Description

    HRP Rabbit polyclonal to ADA

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Species reactivity

    Reacts with: Cow

  • Immunogen

    Adenosine Deaminase (Calf spleen).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

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Properties

Applications

  • Application notes
    Dot: Use at an assay dependent dilution.
    Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/20000 - 1/100000.
    This antibody has been assayed against 1.0µg of Adenosine Deaminase in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 104 kDa.
    Suitable for other peroxidase-antibody based assays.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.

    • Tissue specificity

      Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.

    • Involvement in disease

      Defects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.

    • Sequence similarities

      Belongs to the adenosine and AMP deaminases family.

    • Cellular localization

      Cell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
    • Information by UniProt

    • Alternative names

      • ada antibody
      • ADA_HUMAN antibody
      • ADA1 antibody
      • Adenosine aminohydrolase antibody
      • Adenosine deaminase antibody
      see all

    References (0)

    Publishing research using ab34651? Please let us know so that we can cite the reference in this datasheet.

    ab34651 has not yet been referenced specifically in any publications.

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