RecombinantRabMAb

Recombinant HRP Anti-ADFP antibody [EPR3713] (ab201721)

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Western blot - HRP Anti-ADFP antibody [EPR3713] (ab201721)
  • HRP Anti-ADFP antibody [EPR3713] (ab201721)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR3713] to ADFP
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-ADFP antibody [EPR3713]
    See all ADFP primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR3713] to ADFP

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and Rat brain tissue lysates.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab201721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Rat
All applications
Human
Application Abreviews Notes
WB
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Notes
WB
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

Images

  • Western blot - HRP Anti-ADFP antibody [EPR3713] (ab201721)
    Western blot - HRP Anti-ADFP antibody [EPR3713] (ab201721)
    All lanes : HRP Anti-ADFP antibody [EPR3713] (ab201721) at 5000 µg

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201721 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-ADFP antibody [EPR3713] (ab201721)
    HRP Anti-ADFP antibody [EPR3713] (ab201721)

References (0)

Publishing research using ab201721? Please let us know so that we can cite the reference in this datasheet.

ab201721 has not yet been referenced specifically in any publications.

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