Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR3713] to ADFP
- Suitable for: WB
- Reacts with: Mouse, Rat
- Conjugation: HRP
Product nameHRP Anti-ADFP antibody [EPR3713]
See all ADFP primary antibodies
DescriptionHRP Rabbit monoclonal [EPR3713] to ADFP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Synthetic peptide within Human ADFP aa 1-100. The exact sequence is proprietary.
Database link: Q99541
- WB: Mouse brain and Rat brain tissue lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab201721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
FunctionMay be involved in development and maintenance of adipose tissue.
Tissue specificityMilk lipid globules.
Sequence similaritiesBelongs to the perilipin family.
modificationsAcylated; primarily with C14, C16 and C18 fatty acids.
- Information by UniProt
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
All lanes : HRP Anti-ADFP antibody [EPR3713] (ab201721) at 5000 µg
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201721 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab201721 has not yet been referenced specifically in any publications.