Key features and details
- HRP Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2b
Product nameHRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3]
See all AKR1C1/AKR1C2 primary antibodies
DescriptionHRP Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Full length native protein (purified) corresponding to Human AKR1C1/AKR1C2.
- IHC-P: Normal human liver tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab198411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
- 20-alpha-HSD antibody
- 20-alpha-hydroxysteroid dehydrogenase antibody
IHC image of AKR1C1/AKR1C2 staining in a section of formalin-fixed paraffin-embedded normal human liver*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198411, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab198411 has not yet been referenced specifically in any publications.