HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab198411)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab198411)](https://www.abcam.com/ps/products/198/ab198411/Images/ab198411-238822-IHCap22494591.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab198411)")
Key features and details
- HRP Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3]
See all AKR1C1/AKR1C2 primary antibodies -
Description
HRP Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2 -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
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Immunogen
Full length native protein (purified) corresponding to Human AKR1C1/AKR1C2.
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Positive control
- IHC-P: Normal human liver tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
4B6AF3 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab198411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Database links
- SwissProt: P52895 Human
- SwissProt: Q04828 Human
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Alternative names
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
- 20-alpha-HSD antibody
- 20-alpha-hydroxysteroid dehydrogenase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab198411)
IHC image of AKR1C1/AKR1C2 staining in a section of formalin-fixed paraffin-embedded normal human liver*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198411, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
References (0)
ab198411 has not yet been referenced specifically in any publications.
