Recombinant HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)
![Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)](https://www.abcam.com/ps/products/200/ab200774/Images/ab200774-438757-AntiAldolase--Aldolase-B--Aldolase-C-antibody.jpg "Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR9724(B)] to Aldolase + Aldolase B + Aldolase C
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] -
Description
HRP Rabbit monoclonal [EPR9724(B)] to Aldolase + Aldolase B + Aldolase C -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Aldolase.
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Positive control
- WB : A549 and HeLa whole cell lysates as well as Human fetal liver, fetal brain skeletal muscle tissue lysates. His-tagged human Aldolase A, B and C full-length recombinant protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9724(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab200774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Predicted molecular weight: 39 kDa. |
Images
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Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)All lanes : Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab169544) at 1/1000 dilution
Lane 1 : His-tagged human Aldolase A full-length recombinant protein
Lane 2 : His-tagged human Aldolase B full-length recombinant protein
Lane 3 : His-tagged human Aldolase C full-length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDa -
![Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)](https://www.abcam.com/ps/products/200/ab200774/Images/ab200774-245669-WBab2007741.jpg)
Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)All lanes : HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774) at 1/5000 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : Fetal Liver (Human) Normal Tissue)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Fetal Brain (Human) Normal Tissue lysate
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200774 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)](https://www.abcam.com/ps/products/200/ab200774/Images/ab200774-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)
References (0)
ab200774 has not yet been referenced specifically in any publications.
