Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR9724(B)] to Aldolase + Aldolase B + Aldolase C
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]
DescriptionHRP Rabbit monoclonal [EPR9724(B)] to Aldolase + Aldolase B + Aldolase C
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Aldolase.
- WB : A549 and HeLa whole cell lysates as well as Human fetal liver, fetal brain skeletal muscle tissue lysates. His-tagged human Aldolase A, B and C full-length recombinant protein.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab200774 in the following tested applications.
|WB||1/5000. Predicted molecular weight: 39 kDa.|
All lanes : Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab169544) at 1/1000 dilution
Lane 1 : His-tagged human Aldolase A full-length recombinant protein
Lane 2 : His-tagged human Aldolase B full-length recombinant protein
Lane 3 : His-tagged human Aldolase C full-length recombinant protein
Lysates/proteins at 0.01 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDa
All lanes : HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774) at 1/5000 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : Fetal Liver (Human) Normal Tissue)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Fetal Brain (Human) Normal Tissue lysate
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200774 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab200774 has not yet been referenced specifically in any publications.