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    hrp-aldolase-antibody-ab34598.pdf

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Signal Transduction Metabolism Energy Metabolism
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HRP Anti-Aldolase antibody (ab34598)

  • Datasheet
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Western blot - HRP Anti-Aldolase antibody (ab34598)

    Key features and details

    • HRP Goat polyclonal to Aldolase
    • Suitable for: WB
    • Reacts with: Rabbit, Human
    • Conjugation: HRP
    • Isotype: IgG

    Overview

    • Product name

      HRP Anti-Aldolase antibody
      See all Aldolase primary antibodies
    • Description

      HRP Goat polyclonal to Aldolase
    • Host species

      Goat
    • Conjugation

      HRP
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Rabbit, Human
    • Immunogen

      Full length Aldolase protein (Rabbit muscle).

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      IgG fraction
    • Purification notes

      This antibody was purified by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of carbohydrates
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Carbohydrate metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Amino acid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Positive Controls

      • HEK293 whole cell lysate (ab7902)
    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    Applications

    Our Abpromise guarantee covers the use of ab34598 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
  • Application notes
    Dot: Use at an assay dependent dilution.
    Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/2000.
    WB: 1/1000. Predicted molecular weight: 39 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
    • Pathway

      Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
    • Involvement in disease

      Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
    • Sequence similarities

      Belongs to the class I fructose-bisphosphate aldolase family.
    • Target information above from: UniProt accession P04075 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 226 Human
      • Entrez Gene: 100009055 Rabbit
      • Omim: 103850 Human
      • SwissProt: P04075 Human
      • SwissProt: P00883 Rabbit
      • Unigene: 513490 Human
      • Alternative names

        • ALDA antibody
        • Aldo1 antibody
        • ALDOA antibody
        • ALDOA_HUMAN antibody
        • Aldolase 1 antibody
        • Aldolase A antibody
        • Aldolase A fructose bisphosphatase antibody
        • Aldolase A fructose bisphosphate antibody
        • Aldolase, fructose-bisphosphate A antibody
        • Epididymis secretory sperm binding protein Li 87p antibody
        • FRUCTOALDOLASE A antibody
        • Fructose 1 6 bisphosphate triosephosphate lyase antibody
        • Fructose bisphosphate aldolase A antibody
        • Fructose bisphosphate aldolase antibody
        • FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A antibody
        • Fructose-bisphosphate aldolase A antibody
        • Fructose-bisphosphate aldolase A Muscle-type antibody
        • GSD12 antibody
        • HEL S 87p antibody
        • Lung cancer antigen NY LU 1 antibody
        • Lung cancer antigen NY-LU-1 antibody
        • MGC10942 antibody
        • MGC17716 antibody
        • MGC17767 antibody
        • Muscle type aldolase antibody
        • Muscle-type aldolase antibody
        • RNALDOG5 antibody
        see all

      Images

      • Western blot - HRP Anti-Aldolase antibody (ab34598)
        Western blot - HRP Anti-Aldolase antibody (ab34598)
        HRP Anti-Aldolase antibody (ab34598) at 1/1000 dilution + A293 cell lysate

        Predicted band size: 39 kDa
        Observed band size: 41 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab34598? Please let us know so that we can cite the reference in this datasheet.

      ab34598 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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