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    hrp-alpha-lactalbumin-antibody-ab112973.pdf

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HRP Anti-alpha Lactalbumin antibody (ab112973)

  • Datasheet
  • SDS
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Abpromise

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Key features and details

  • HRP Goat polyclonal to alpha Lactalbumin
  • Reacts with: Cow
  • Conjugation: HRP
  • Isotype: IgG

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Anti-alpha Lactalbumin antibody (ab112972)
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Anti-alpha Lactalbumin antibody [EPR12461] - BSA and Azide free (ab250182)

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Overview

  • Product name

    HRP Anti-alpha Lactalbumin antibody
    See all alpha Lactalbumin primary antibodies
  • Description

    HRP Goat polyclonal to alpha Lactalbumin
  • Host species

    Goat
  • Conjugation

    HRP
  • Species reactivity

    Reacts with: Cow
  • Immunogen

    Bovine alpha-lactalbumin isolated from whole normal bovine serum

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure specificity. ab112973 was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Immunology
    • Secreted Molecules
    • Other secreted molecules

Associated products

  • Recombinant Protein

    • Recombinant Human alpha Lactalbumin protein (His tag) (ab185402)
  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Target

  • Function

    Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
  • Tissue specificity

    Mammary gland specific. Secreted in milk.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 22 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P00709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281894 Cow
    • SwissProt: P00711 Cow
    • Alternative names

      • a-LACTA antibody
      • alfaLA antibody
      • Alpha-lactalbumin antibody
      • Lactalbumin alpha antibody
      • Lactose synthase B protein antibody
      • LALBA antibody
      • LALBA_HUMAN antibody
      • Lysozyme-like protein 7 antibody
      • LYZL7 antibody
      • MGC138521 antibody
      • MGC138523 antibody
      see all

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab112973? Please let us know so that we can cite the reference in this datasheet.

    ab112973 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Dear Sir/Madam The customer will set ELISA application to detect the above 4 protein from human breast milk. 1. Beta-lactoglobulin : AB8226, AB8227 2. Alpha-lactalbumin : AB20212,AB112972, AB112973 3. Casein : AB47972 4. Lactoferrin : AB108882 Please kindly recommend technical or protocol and ab items No. suitable.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 09 2011

    Answer

    Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ortholog, and to my knowledge, in most experimental settings the bovine protein is detected in human breast milk (e.g. PMID8163788). If this is of interest for the customer, I am happy to let you know that we have two antibodies against the bovine beta-lactoglobulin in our catalog (ab112893, ab112894). Click here (or use the following: https://www.abcam.com/LGB-antibody-ab112893.html). Click here (or use the following: https://www.abcam.com/HRP-LGB-antibody-ab112894.html). 2) Alpha-lactalbumin: Again, we have antibodies against the bovine protein, but also one antibody against the human protein which are guaranteed to work in ELISA (ab112972, ab112973, ab35288). Click here (or use the following: https://www.abcam.com/alpha-Lactalbumin-antibody-ab112972.html). Click here (or use the following: https://www.abcam.com/HRP-alpha-Lactalbumin-antibody-ab112973.html). Click here (or use the following: https://www.abcam.com/alpha-Lactalbumin-antibody-ab35288.html). 3) Casein: We only have one antibody against the human Casein, suitable for ELISA (ab91167). Click here (or use the following: https://www.abcam.com/Casein-antibody-ab91167.html). 4) Lactoferrin: We have a number of antibodies available against the human lactoferrin some of which are not only guaranteed to work in a conventional ELISA, but also in Sandwich ELISA (sELISA), for example ab10110 or ab15811. Click here (or use the following: https://www.abcam.com/Lactoferrin-antibody-2B8-ab10110.html). Click here (or use the following: https://www.abcam.com/Lactoferrin-antibody-ab15811.html). Please find attached our ELISA protocols. If the customer is interested in matched antibody pairs for Sandwich ELISA, or antibodies against these targets from other species than human, I would be pleased to assist in finding the most suitable ones. Please be reassured that all antibodies are covered by our Abpromise: We are happy to offer technical support, and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. All tested species and applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. If the customer decides to go ahead and test these products in an untested species or application, please contact our Scientific Support team prior to the purchase as they might be eligible for our testing discount program. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Abcam Scientific Support

    Answered on Dec 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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