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  1. Link

    hrp-alpha-smooth-muscle-actin-antibody-1a4-ab203696.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Validated using a knockout cell line

HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
  • Western blot - HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
  • Western blot - HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)

Key features and details

  • HRP Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG2a

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Overview

  • Product name

    HRP Anti-alpha smooth muscle Actin antibody [1A4]
    See all alpha smooth muscle Actin primary antibodies
  • Description

    HRP Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Pig, Mammals, Baboon
  • Immunogen

    Synthetic peptide corresponding to Human alpha smooth muscle Actin (N terminal).
    Database link: P62736

  • Positive control

    • WB: A431 and Jurkat whole cell lines. IHC-P: Normal human colon tissue sections
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    1A4
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

Associated products

  • Alternative Versions

    • Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)
    • Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
    • Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
    • Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
    • Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    • FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
  • KO cell lines

    • Human ACTA2 knockout HeLa cell line (ab264014)
  • KO cell lysates

    • Human ACTA2 knockout HeLa cell lysate (ab264499)
  • Recombinant Protein

    • Recombinant Human alpha smooth muscle Actin protein (ab134555)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P62736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 101021287 Baboon
    • Entrez Gene: 515610 Cow
    • Entrez Gene: 59 Human
    • Entrez Gene: 11475 Mouse
    • Entrez Gene: 733615 Pig
    • Entrez Gene: 100009271 Rabbit
    • Entrez Gene: 81633 Rat
    • Entrez Gene: 101121051 Sheep
    • Omim: 102620 Human
    • SwissProt: P62739 Cow
    • SwissProt: P62736 Human
    • SwissProt: P62737 Mouse
    • SwissProt: P62740 Rabbit
    • SwissProt: P62738 Rat
    • Unigene: 500483 Human
    • Unigene: 213025 Mouse
    • Unigene: 195319 Rat
    • Unigene: 3114 Rat
    see all
  • Alternative names

    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)

    IHC image of alpha smooth muscle Actin staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab203696, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
    Western blot - HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
    All lanes : HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696) at 1/5000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ACTA2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 20 seconds


    ab203696 was shown to react with alpha smooth muscle Actin (HRP) in HeLa wild-type cells in western blot. Loss of signal was observed when ACTA2 knockout sample was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab203696 overnight at 4°C at a 1 in 5000 Dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.

  • Western blot - HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
    Western blot - HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
    All lanes : HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203696 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab203696? Please let us know so that we can cite the reference in this datasheet.

ab203696 has not yet been referenced specifically in any publications.

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