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  1. Link

    hrp-alpha-tubulin-antibody-dm1a-loading-control-ab40742.pdf

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Isotype/Loading Controls Loading Controls Tubulin
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HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (50)

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Western blot - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)

Key features and details

  • HRP Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Conjugation: HRP
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control
    See all alpha Tubulin primary antibodies
  • Description

    HRP Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Xenopus laevis
    Predicted to work with: Guinea pig
  • Immunogen

    Full length native protein (purified) corresponding to Chicken alpha Tubulin.

  • Positive control

    • This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Brain (Rat) Tissue Lysate - normal tissue Xenopus (Blastula) Whole Cell Lysate. IHC-P: FFPE human colon (normal) tissue sections.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    DM1A
  • Isotype

    IgG
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Tubulin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Alpha Tubulin
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093)
    • Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
    • Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)
    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
    • Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free (ab264493)
    • FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)
    • alpha Tubulin peptide (ab195584)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (5)
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7277 Human
    • Entrez Gene: 22145 Mouse
    • Entrez Gene: 316531 Rat
    • Omim: 191110 Human
    • SwissProt: P68366 Human
    • SwissProt: P68368 Mouse
    • SwissProt: Q5XIF6 Rat
    • Unigene: 75318 Human
    • Unigene: 1155 Mouse
    • Unigene: 92961 Rat
    see all
  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Western blot - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
    Western blot - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
    All lanes : HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH/3T3 whole cell lysate (ab7179)
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Xenopus Laevis Protein Extracts (Stg 8)

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)

    IHC image of alpha Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab40742, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (50)

Publishing research using ab40742? Please let us know so that we can cite the reference in this datasheet.

ab40742 has been referenced in 50 publications.

  • Jansens RJJ  et al. Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex. Cell Rep 40:111107 (2022). WB, IP, ICC ; Pig . PubMed: 35858564
  • Hasegawa Y  et al. Identification and Analysis of a Novel NR0B1 Mutation in Late-Onset Adrenal Hypoplasia Congenita and Hypogonadism. J Endocr Soc 5:bvaa176 (2021). PubMed: 33381670
  • Li Y  et al. Hedgehog interacting protein (HHIP) represses airway remodeling and metabolic reprogramming in COPD-derived airway smooth muscle cells. Sci Rep 11:9074 (2021). PubMed: 33907231
  • Shaik S  et al. REST promotes ETS1-dependent vascular growth in medulloblastoma. Mol Oncol 15:1486-1506 (2021). PubMed: 33469989
  • Tsuruhisa S  et al. Pigment epithelium-derived factor inhibits advanced glycation end product-induced proliferation, VEGF and MMP-9 expression in breast cancer cells via interaction with laminin receptor. Oncol Lett 22:629 (2021). PubMed: 34267821
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP)

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Baboon Tissue lysate - whole (Fetal Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
12.5 µg
Specification
Fetal Skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Soon-Ok Kim

Verified customer

Submitted Feb 09 2017

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Chicken Tissue lysate - whole (Chicken Embryo)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Chicken Embryo
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Yuval Cinnamon

Verified customer

Submitted Dec 12 2016

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP)

Inconclusive
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 12 2015

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Neonatal Rat cardiomyocytes)
Loading amount
20 µg
Specification
Neonatal Rat cardiomyocytes
Gel Running Conditions
Reduced Non-Denaturing (Native) (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 30 2011

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] (HRP)

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
50 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (8% Tris/HCL)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 28 2011

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