Anti-HRP antibody [2H11] (ab10183)

Submit a review Q&A (1)References (5)

Key features and details

  • Mouse monoclonal [2H11] to HRP
  • Isotype: IgG2b

Overview

  • Product name

    Anti-HRP antibody [2H11]
    See all HRP primary antibodies

  • Description

    Mouse monoclonal [2H11] to HRP

  • Host species

    Mouse

  • Specificity

    Horseradish peroxidase isoforms with pI from 3.5 to 9.5. ab10183 does not influence enzymatic activity upon immune complex formation.

  • Immunogen

    Purified peroxidase from horseradish.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

  • Clonality

    Monoclonal

  • Clone number

    2H11

  • Myeloma

    Sp2/0

  • Isotype

    IgG2b

  • Research areas

Target

  • Relevance

    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

  • Cellular localization

    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.

  • Alternative names

    • Horseradish Peroxidase antibody
    • HPRC1 antibody
    • HRP antibody
    • Peroxidase antibody
    • Peroxidase C1A antibody
    • PRXC1A antibody
    see all

References (5)

Publishing research using ab10183? Please let us know so that we can cite the reference in this datasheet.

ab10183 has been referenced in 5 publications.

  • Liu B  et al. Effect of microRNA-27b on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma via FZD7 signaling pathway. Oncol Lett 18:667-673 (2019). PubMed: 31289540
  • Lin TY  et al. Mapping chromatic pathways in the Drosophila visual system. J Comp Neurol 524:213-27 (2016). PubMed: 26179639
  • Shazeeb MS  et al. Targeted signal-amplifying enzymes enhance MRI of EGFR expression in an orthotopic model of human glioma. Cancer Res 71:2230-9 (2011). IHC-Fr . PubMed: 21245103
  • Terashima H  et al. A sensitive short-term evaluation of antifibrotic effects using newly established type I collagen reporter transgenic rats. Am J Physiol Renal Physiol 299:F792-801 (2010). IHC-Fr . PubMed: 20660018
  • He DF  et al. Morpho-Physiologic Characteristics of Dorsal Subicular Network in Mice after Pilocarpine-Induced Status Epilepticus. Brain Pathol : (2009). IHC . PubMed: 19298597

Customer reviews and Q&As

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Question

Does ab10183 antibody like most other (polyclonal) antibodies against HRP only recognize the N-glycan on the HRP protein backbone?

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Answer

Thank you for your enquiry. Unfortunately, the HRP epitope that this antibody recognizes has not been determined. However, it is likely to recognizes the protein part of the molecule because it reacts with various peroxidase strains differing in composition of carbohydrate part. The peroxidase retains its activity after linking to the antibody. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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