Anti-HRP antibody [2H11] (ab10183)
Key features and details
- Mouse monoclonal [2H11] to HRP
- Suitable for: ELISA, IHC-Fr, IHC-P
- Isotype: IgG2b
Overview
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Product name
Anti-HRP antibody [2H11]
See all HRP primary antibodies -
Description
Mouse monoclonal [2H11] to HRP -
Host species
Mouse -
Specificity
Horseradish peroxidase isoforms with pI from 3.5 to 9.5. ab10183 does not influence enzymatic activity upon immune complex formation. -
Tested applications
Suitable for: ELISA, IHC-Fr, IHC-Pmore details -
Immunogen
Full length native protein (purified) corresponding to HRP.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis. -
Clonality
Monoclonal -
Clone number
2H11 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. -
Cellular localization
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles. -
Alternative names
- Horseradish Peroxidase antibody
- HPRC1 antibody
- HRP antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab10183 has been referenced in 9 publications.
- Zhang B et al. Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2. Aging (Albany NY) 13:2941-2958 (2021). PubMed: 33460397
- Wang Z et al. Long noncoding RNA PTENP1 affects the recovery of spinal cord injury by regulating the expression of miR-19b and miR-21. J Cell Physiol 235:3634-3645 (2020). PubMed: 31583718
- Sharafeldin M et al. Influence of antibody immobilization strategy on carbon electrode immunoarrays. Analyst 144:5108-5116 (2019). PubMed: 31373337
- Liu B et al. Effect of microRNA-27b on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma via FZD7 signaling pathway. Oncol Lett 18:667-673 (2019). PubMed: 31289540
- Lin TY et al. Mapping chromatic pathways in the Drosophila visual system. J Comp Neurol 524:213-27 (2016). PubMed: 26179639
- Miyazaki T et al. A gustatory second-order neuron that connects sucrose-sensitive primary neurons and a distinct region of the gnathal ganglion in the Drosophila brain. J Neurogenet 29:144-55 (2015). PubMed: 26004543
- Shazeeb MS et al. Targeted signal-amplifying enzymes enhance MRI of EGFR expression in an orthotopic model of human glioma. Cancer Res 71:2230-9 (2011). IHC-Fr . PubMed: 21245103
- Terashima H et al. A sensitive short-term evaluation of antifibrotic effects using newly established type I collagen reporter transgenic rats. Am J Physiol Renal Physiol 299:F792-801 (2010). IHC-Fr . PubMed: 20660018
- He DF et al. Morpho-Physiologic Characteristics of Dorsal Subicular Network in Mice after Pilocarpine-Induced Status Epilepticus. Brain Pathol : (2009). IHC . PubMed: 19298597