Overview

  • Product name
    Anti-HRP antibody [2H11]
    See all HRP primary antibodies
  • Description
    Mouse monoclonal [2H11] to HRP
  • Host species
    Mouse
  • Specificity
    Horseradish peroxidase isoforms with pI from 3.5 to 9.5. ab10183 does not influence enzymatic activity upon immune complex formation.
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Frmore details
  • Immunogen

    Purified peroxidase from horseradish.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    2H11
  • Myeloma
    Sp2/0
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
  • Cellular localization
    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
  • Alternative names
    • Horseradish Peroxidase antibody
    • HPRC1 antibody
    • HRP antibody
    • Peroxidase antibody
    • Peroxidase C1A antibody
    • PRXC1A antibody
    see all

References

This product has been referenced in:
  • Shazeeb MS  et al. Targeted signal-amplifying enzymes enhance MRI of EGFR expression in an orthotopic model of human glioma. Cancer Res 71:2230-9 (2011). IHC-Fr . Read more (PubMed: 21245103) »
  • Terashima H  et al. A sensitive short-term evaluation of antifibrotic effects using newly established type I collagen reporter transgenic rats. Am J Physiol Renal Physiol 299:F792-801 (2010). IHC-Fr . Read more (PubMed: 20660018) »

See all 3 Publications for this product

Publishing research using ab10183? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

Does ab10183 antibody like most other (polyclonal) antibodies against HRP only recognize the N-glycan on the HRP protein backbone?

Thank you for your enquiry. Unfortunately, the HRP epitope that this antibody recognizes has not been determined. However, it is likely to recognizes the protein part of the molecule because it reacts with various peroxidase strains differing in com...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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