Anti-HRP antibody [2H11] (ab10183)

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Overview

  • Product name

    Anti-HRP antibody [2H11]
    See all HRP primary antibodies

  • Description

    Mouse monoclonal [2H11] to HRP

  • Host species

    Mouse

  • Specificity

    Horseradish peroxidase isoforms with pI from 3.5 to 9.5. ab10183 does not influence enzymatic activity upon immune complex formation.

  • Tested applications

    Suitable for: ELISA, IHC-P, IHC-Frmore details

  • Immunogen

    Purified peroxidase from horseradish.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

  • Clonality

    Monoclonal

  • Clone number

    2H11

  • Myeloma

    Sp2/0

  • Isotype

    IgG2b

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance

    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

  • Cellular localization

    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.

  • Alternative names

    • Horseradish Peroxidase antibody
    • HPRC1 antibody
    • HRP antibody
    • Peroxidase antibody
    • Peroxidase C1A antibody
    • PRXC1A antibody
    see all

References

This product has been referenced in:

  • Liu B  et al. Effect of microRNA-27b on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma via FZD7 signaling pathway. Oncol Lett 18:667-673 (2019). Read more (PubMed: 31289540) »
  • Shazeeb MS  et al. Targeted signal-amplifying enzymes enhance MRI of EGFR expression in an orthotopic model of human glioma. Cancer Res 71:2230-9 (2011). IHC-Fr . Read more (PubMed: 21245103) »
See all 4 Publications for this product

Publishing research using ab10183? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Does ab10183 antibody like most other (polyclonal) antibodies against HRP only recognize the N-glycan on the HRP protein backbone?

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Answer

Thank you for your enquiry. Unfortunately, the HRP epitope that this antibody recognizes has not been determined. However, it is likely to recognizes the protein part of the molecule because it reacts with various peroxidase strains differing in composition of carbohydrate part. The peroxidase retains its activity after linking to the antibody. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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