Anti-HRP antibody (ab181658)
Key features and details
- Goat polyclonal to HRP
- Suitable for: WB
- Reacts with: Horseradish
- Isotype: IgG
Overview
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Product name
Anti-HRP antibody
See all HRP primary antibodies -
Description
Goat polyclonal to HRP -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Horseradish -
Immunogen
Full length native protein (purified) corresponding to HRP. (Horseradish).
Database link: P00433 -
Positive control
- Horseradish peroxidase
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
ab181658 was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 39 kDa.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 39 kDa. |
Target
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Relevance
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. -
Cellular localization
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles. -
Database links
- SwissProt: P00433 Horseradish
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Alternative names
- Horseradish Peroxidase antibody
- HPRC1 antibody
- HRP antibody
see all
Images
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All lanes : Anti-HRP antibody (ab181658) at 1/5000 dilution
Lane 1 : Horseradish peroxidase (reducing conditions) at 1 µg
Lane 2 : Horseradish peroxidase (reducing conditions) at 0.25 µg
Lane 3 : Horseradish peroxidase (non-reducing conditions) at 1 µg
Lane 4 : Horseradish peroxidase (non-reducing conditions) at 0.25 µg
Secondary
All lanes : Dylight 649 conjugated Donkey anti Goat at 1/10000 dilution
Predicted band size: 39 kDaReduced samples of purified Horseradish peroxidase contained 4% BME and were boiled for 5 minutes.
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Anti-HRP antibody (ab181658) at 1/1000 dilution + Peroxidase at 0.05 µg
Secondary
DyLight™ 649 goat secondary at 1/20000 dilution
Predicted band size: 39 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab181658 has been referenced in 23 publications.
- Liang X et al. Dickkopf‑3 and β‑catenin play opposite roles in the Wnt/β‑catenin pathway during the abnormal subchondral bone formation of human knee osteoarthritis. Int J Mol Med 49:N/A (2022). PubMed: 35137918
- Long J et al. miR-500a-3p is a Potential Prognostic Biomarker in Hepatocellular Carcinoma. Int J Gen Med 15:1891-1899 (2022). PubMed: 35221718
- Tan Y et al. Circular RNA ZFR promotes cell cycle arrest and apoptosis of colorectal cancer cells via the miR-147a/CACUL1 axis. J Gastrointest Oncol 13:1793-1804 (2022). PubMed: 36092343
- Sun M et al. Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS-induced BV2 cells in vitro and in vivo. Exp Ther Med 21:586 (2021). PubMed: 33850558
- Liu W & Liu X LINC00641 Inhibits the Development of Cutaneous Squamous Cell Carcinoma By Downregulating miR-424 in A431 Cells. Cancer Biother Radiopharm N/A:N/A (2021). PubMed: 33885353