Overview

  • Product name

  • Description

    Goat polyclonal to HRP
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Horseradish
  • Immunogen

    Full length native protein (purified) corresponding to HRP. (Horseradish).
    Database link: P00433

  • Positive control

    • Horseradish peroxidase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    ab181658 was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000.
WB 1/2000 - 1/10000. Predicted molecular weight: 39 kDa.

Target

  • Relevance

    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
  • Cellular localization

    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
  • Database links

    • Alternative names

      • Horseradish Peroxidase antibody
      • HPRC1 antibody
      • HRP antibody
      • Peroxidase antibody
      • Peroxidase C1A antibody
      • PRXC1A antibody
      see all

    Images

    • All lanes : Anti-HRP antibody (ab181658) at 1/5000 dilution

      Lane 1 : Horseradish peroxidase (reducing conditions) at 1 µg
      Lane 2 : Horseradish peroxidase (reducing conditions) at 0.25 µg
      Lane 3 : Horseradish peroxidase (non-reducing conditions) at 1 µg
      Lane 4 : Horseradish peroxidase (non-reducing conditions) at 0.25 µg

      Secondary
      All lanes : Dylight 649 conjugated Donkey anti Goat at 1/10000 dilution

      Predicted band size: 39 kDa



      Reduced samples of purified Horseradish peroxidase contained 4% BME and were boiled for 5 minutes.

    • Anti-HRP antibody (ab181658) at 1/1000 dilution + Peroxidase at 0.05 µg

      Secondary
      DyLight™ 649 goat secondary at 1/20000 dilution

      Predicted band size: 39 kDa

    References

    This product has been referenced in:

    • Huang Q  et al. Incremental effect of liraglutide on traditional insulin injections in rats with type 2 diabetes mellitus by maintaining glycolipid metabolism and cardiovascular function. Exp Ther Med 17:1863-1869 (2019). Read more (PubMed: 30783461) »
    • Zhao H  et al. LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway. Oncol Lett 15:6839-6844 (2018). Read more (PubMed: 29731863) »
    See all 7 Publications for this product

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