Key features and details
- Mouse polyclonal to HRP
- Suitable for: WB
- Reacts with: Horseradish
- Isotype: IgG
Product nameAnti-HRP antibody
See all HRP primary antibodies
DescriptionMouse polyclonal to HRP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Horseradish
Full length native protein (purified) corresponding to HRP aa 31-338.
QLTPTFYDNSCPNVSNIVRDTIVNELRSDPRIAASILRLHFHDCFVNGCD ASILLDNTTSFRTEKDAFGNANSARGFPVIDRMKAAVESACPRTVSCADL LTIAAQQSVTLAGGPSWRVPLGRRDSLQAFLDLANANLPAPFFTLPQLKD SFRNVGLNRSSDLVALSGGHTFGKNQCRFIMDRLYNFSNTGLPDPTLNTT YLQTLRGLCPLNGNLSALVDFDLRTPTIFDNKYYVNLEEQKGLIQSDQEL FSSPNATDTIPLVRSFANSTQTFFNAFVEAMDRMGNITPLTGTQGQIRLN CRVVNSNS
Database link: P00433
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituents: 0.88% Sodium chloride, 0.27% Potassium phosphate
Does not contain preservative or stabilizer
Concentration information loading...
Purification notesab181708 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the stated buffer stated. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum as well as purified and partially purified Peroxidase [Horseradish].
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab181708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 39 kDa.|
RelevanceHorseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Cellular localizationSecreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
- SwissProt: P00433 Horseradish
- Horseradish Peroxidase antibody
- HPRC1 antibody
- HRP antibody
Anti-HRP antibody (ab181708) at 1/1000 dilution (overnight at 4°C) + Peroxidase (Horseradish) at 100 µg
Fluorescein conjugated mouse secondary antibody for 30 minutes at room temperature at 1/40000 dilution
Predicted band size: 39 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Block: Blocking Buffer for Fluorescent Western Blotting for 30 minutes at room temperature.
ab181708 has not yet been referenced specifically in any publications.