Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
  • Cellular localization
    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
  • Alternative names
    • Horseradish Peroxidase antibody
    • HPRC1 antibody
    • HRP antibody
    • Peroxidase antibody
    • Peroxidase C1A antibody
    • PRXC1A antibody
    see all

References

ab2115 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

I have been in contact with the lab regarding this issue and have been informed that this product is not a purified IgG fraction as stated on the datasheet but is instead whole antiserum. This would explain the results which you have seen as the antiserum would contain other proteins as well. We are correcting this information on our datasheets presently.

We believe that this issue arose as we offer the IgG purified version of this product which has an internal designation number which is very similar to this product's number. The purified version of this anti-HRP is ab2115.

As this product is not as we have stated on our datasheet I may offer you replacement of this product should you wish. Please let me know if would like me to replace this product for you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your inquiry and for the interesting information. After investigation with my colleagues, I have been informed we do not have any data that our anti HRP polyclonal antibody react with alpha 1,3 fucosyl and/or beta 1,2 xylosyl sugars on the glycosylation structure as we do not test our anti HRP antibody for such reactivity. Unfortunately, I can confirm we have no anti-plant glycan antibodies available in our catalogue. I am sorry I am unable to suggest a suitable antibody for you on this occasion. I can suggest having a look on the Biocompare website. They have an excellent antibody search facility which includes a lot of suppliers. I hope this helps. Should you have any further questions, please do not hesitate to contact us. Good luck with your search.

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Answer

We do not have any reports of any of our HRP antibodies being used as a primary antibody with frozen or paraffin embedded sections. In addition, cross-reactivity with endogenous tissue peroxidases has not been tested.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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