Anti-HRP antibody (ab2116)
Key features and details
- Sheep polyclonal to HRP
- Suitable for: ELISA, Conjugation, IP, WB
- Reacts with: Horseradish
- Isotype: IgG
Overview
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Product name
Anti-HRP antibody
See all HRP primary antibodies -
Description
Sheep polyclonal to HRP -
Host species
Sheep -
Tested applications
Suitable for: ELISA, Conjugation, IP, WBmore details -
Species reactivity
Reacts with: Horseradish -
Immunogen
Full length native protein (purified) corresponding to HRP. Horseradish peroxidase
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Conjugation |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Conjugation
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Relevance
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. -
Cellular localization
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles. -
Database links
- SwissProt: P00433 Horseradish
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Alternative names
- Horseradish Peroxidase antibody
- HPRC1 antibody
- HRP antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab2116 has been referenced in 1 publication.
- Li YF et al. Targeting MRP4 expression by anti-androgen treatment reverses MRP4-mediated docetaxel resistance in castration-resistant prostate cancer. Oncol Lett 14:1748-1756 (2017). PubMed: 28789405