Anti-HRP antibody (ab2116)

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Key features and details

  • Sheep polyclonal to HRP
  • Isotype: IgG

Overview

  • Product name

    Anti-HRP antibody
    See all HRP primary antibodies

  • Description

    Sheep polyclonal to HRP

  • Host species

    Sheep

  • Immunogen

    Horseradish peroxidase

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    IgG fraction

  • Purification notes

    IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Target

  • Relevance

    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

  • Cellular localization

    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.

  • Alternative names

    • Horseradish Peroxidase antibody
    • HPRC1 antibody
    • HRP antibody
    • Peroxidase antibody
    • Peroxidase C1A antibody
    • PRXC1A antibody
    see all

References (1)

Publishing research using ab2116? Please let us know so that we can cite the reference in this datasheet.

ab2116 has been referenced in 1 publication.

  • Li YF  et al. Targeting MRP4 expression by anti-androgen treatment reverses MRP4-mediated docetaxel resistance in castration-resistant prostate cancer. Oncol Lett 14:1748-1756 (2017). PubMed: 28789405

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

is this antiserum suitable for inhibiting peroxidase activity?

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Answer

The antibody has not been tested for inhibition of peroxidase. We have introduced a new scheme to allow researchers to try an antibody in an unknown application 'risk free'. If you want to place an order with our reference LD/NS.02.08.01 we can include the antibody in this scheme (No#2 below applies in this case). 1.) Abcam will refund the product if it does not work in the application and species as stated on the datasheet. 2.) If it is used in an application or on a species that is not listed on the datasheet then Abcam will refund or replace the product for another of equal value as long as Abcam receives the negative data for publishing on the datasheets to help other researchers.

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Question

I have tumours grown using transfectants stably expressing the HRP enzyme and would like to stain using immunohistochemistry. Are any of your horseradish peroxidase antibodies suitable for IHC in either frozen or paraffin embeded sections, and would they also pick up other peroxidases present in the tissue such as myeloperoxidase?

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Answer

We do not have any reports of any of our HRP antibodies being used as a primary antibody with frozen or paraffin embedded sections. In addition, cross-reactivity with endogenous tissue peroxidases has not been tested.

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