Anti-HRP antibody (ab2116)

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Overview

  • Product name
    Anti-HRP antibody
    See all HRP primary antibodies
  • Description
    Sheep polyclonal to HRP
  • Host species
    Sheep
  • Tested applications
    Suitable for: WB, IP, ELISA, Conjugationmore details
  • Immunogen

    Horseradish peroxidase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
Conjugation
  • Application notes
    Optimal titers for applications should be determined by the researcher.

    • Target

    • Relevance
      Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
    • Cellular localization
      Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
    • Alternative names
      • Horseradish Peroxidase antibody
      • HPRC1 antibody
      • HRP antibody
      • Peroxidase antibody
      • Peroxidase C1A antibody
      • PRXC1A antibody
      see all

    References

    This product has been referenced in:
    • Li YF  et al. Targeting MRP4 expression by anti-androgen treatment reverses MRP4-mediated docetaxel resistance in castration-resistant prostate cancer. Oncol Lett 14:1748-1756 (2017). Read more (PubMed: 28789405) »
    See 1 Publication for this product

    Publishing research using ab2116? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    is this antiserum suitable for inhibiting peroxidase activity?

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    Answer

    The antibody has not been tested for inhibition of peroxidase. We have introduced a new scheme to allow researchers to try an antibody in an unknown application 'risk free'. If you want to place an order with our reference LD/NS.02.08.01 we can include the antibody in this scheme (No#2 below applies in this case). 1.) Abcam will refund the product if it does not work in the application and species as stated on the datasheet. 2.) If it is used in an application or on a species that is not listed on the datasheet then Abcam will refund or replace the product for another of equal value as long as Abcam receives the negative data for publishing on the datasheets to help other researchers.

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    Question

    I have tumours grown using transfectants stably expressing the HRP enzyme and would like to stain using immunohistochemistry. Are any of your horseradish peroxidase antibodies suitable for IHC in either frozen or paraffin embeded sections, and would they also pick up other peroxidases present in the tissue such as myeloperoxidase?

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    Answer

    We do not have any reports of any of our HRP antibodies being used as a primary antibody with frozen or paraffin embedded sections. In addition, cross-reactivity with endogenous tissue peroxidases has not been tested.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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