Anti-HRP antibody (ab34961)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-HRP antibody
See all HRP primary antibodies -
DescriptionMouse polyclonal to HRP
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Host speciesMouse
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with Horseradish.
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Immunogen
Full length Horseradish peroxidase.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate -
PurityWhole antiserum
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Purification notesThis product was prepared from monospecific antiserum by delipidation and defibrination.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
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- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
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- 450 nm Stop Solution for TMB Substrate (ab171529)
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Applications
Our Abpromise guarantee covers the use of ab34961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 35 kDa. | |
| ELISA | 1/5000 - 1/20000. |
Target
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RelevanceHorseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
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Cellular localizationSecreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
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Alternative names
- Horseradish Peroxidase antibody
- HPRC1 antibody
- HRP antibody
see all
Protocols
Datasheets and documents
References
This product has been referenced in:
- Li P et al. MicroRNA-18a regulates invasive meningiomas via hypoxia-inducible factor-1a. Exp Ther Med 10:1165-1170 (2015). WB . Read more (PubMed: 26622458) »
