Application
ELISA
Sample
Human Recombinant protein (human recombinant protein were used)
Specification
human recombinant protein were used
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Direct
Other product details
Dilution
1/4000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA in PBS
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
I use a GST recombinant protein on ELISA and compare this antibody to GST conjugated HRP. The sensitivity of this antibody is about 5X less. But it does provide specific positive response on ELISA.
Abcam response
To increase the signal, Abcam recommends increasing the primary incubation time to overnight at 4C.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Mar 12 2008