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    hrp-apx2-antibody-ab192968.pdf

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HRP Anti-APX2 antibody (ab192968)

  • Datasheet
  • SDS
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Western blot - HRP Anti-APX2 antibody (ab192968)

    Key features and details

    • HRP Rabbit polyclonal to APX2
    • Suitable for: WB
    • Reacts with: Arabidopsis thaliana
    • Conjugation: HRP
    • Isotype: IgG

    Overview

    • Product name

      HRP Anti-APX2 antibody
      See all APX2 primary antibodies
    • Description

      HRP Rabbit polyclonal to APX2
    • Host species

      Rabbit
    • Conjugation

      HRP
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Arabidopsis thaliana
    • Immunogen

      Recombinant full length protein corresponding to Arabidopsis thaliana APX2 aa 4-250.
      Database link: Q1PER6

    • General notes

       This product was previously labelled as L-ascorbate peroxidase 2, L-ascorbate peroxidase 2

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze.
    • Storage buffer

      pH: 7.40
      Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      >95%,Protein G purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Other Antibodies
      • Plant related targets
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress

    Applications

    Our Abpromise guarantee covers the use of ab192968 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/5000. Predicted molecular weight: 28 kDa.

    Target

    • Function

      Plays a key role in hydrogen peroxide removal.
    • Tissue specificity

      Detected in bundle sheath cells, the photosynthetic cells that surround the phloem and xylem.
    • Sequence similarities

      Belongs to the peroxidase family. Ascorbate peroxidase subfamily.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q1PER6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 820121 Arabidopsis thaliana
      • SwissProt: Q1PER6 Arabidopsis thaliana
      • Unigene: 129 Arabidopsis thaliana
      • Alternative names

        • APX1b antibody
        • APX2 antibody
        • APX2_ARATH antibody
        • AtAPx02 antibody
        • cytosolic antibody
        • L-ascorbate peroxidase 1b antibody
        • L-ascorbate peroxidase 2 antibody
        see all

      Images

      • Western blot - HRP Anti-APX2 antibody (ab192968)
        Western blot - HRP Anti-APX2 antibody (ab192968)

        Western blot analysis of Arabidopsis thaliana lysate at 80μg, 40μg and 20μg labeling L-ascorbate peroxidase 2 with ab192968 at 0.6µg/ml.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab192968? Please let us know so that we can cite the reference in this datasheet.

      ab192968 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-APX2 antibody (HRP)

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK293T)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      HEK293T
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Dr. Vladimir Zhemkov

      Verified customer

      Submitted Jan 06 2020

      Western blot abreview for Anti-L-ascorbate peroxidase 2 antibody (HRP)

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Soybean Cell lysate - whole cell (HeLa cells transfected with mutated soybean APX)
      Gel Running Conditions
      Reduced Denaturing (12.5)
      Loading amount
      50000 cells
      Specification
      HeLa cells transfected with mutated soybean APX
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 12 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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