RecombinantRabMAb

Recombinant HRP Anti-ASS1 antibody [EPR12398] (ab209018)

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Western blot - HRP Anti-ASS1 antibody [EPR12398] (ab209018)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ASS1 antibody [EPR12398] (ab209018)
  • HRP Anti-ASS1 antibody [EPR12398] (ab209018)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR12398] to ASS1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-ASS1 antibody [EPR12398]
    See all ASS1 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR12398] to ASS1

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human ASS1 aa 1-100. The exact sequence is proprietary.
    Database link: P00966

  • Positive control

    • WB: Human Liver lysate. IHC-P: FFPE normal human liver tissue sections.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab209018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway

    Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
    Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.

  • Involvement in disease

    Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.

  • Sequence similarities

    Belongs to the argininosuccinate synthase family. Type 1 subfamily.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Argininosuccinate synthase 1 antibody
    • Argininosuccinate synthase antibody
    • Argininosuccinate synthetase 1 antibody
    • ASS antibody
    • Ass-1 antibody
    • ass1 antibody
    • ASSA antibody
    • ASSY_HUMAN antibody
    • Citrulline aspartate ligase antibody
    • Citrulline--aspartate ligase antibody
    • CTLN1 antibody
    see all

Images

  • Western blot - HRP Anti-ASS1 antibody [EPR12398] (ab209018)
    Western blot - HRP Anti-ASS1 antibody [EPR12398] (ab209018)
    HRP Anti-ASS1 antibody [EPR12398] (ab209018) + Human liver lysate at 10 µg

    Predicted band size: 47 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking buffer: 2% BSA

    Gel type: MOPS

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ASS1 antibody [EPR12398] (ab209018)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ASS1 antibody [EPR12398] (ab209018)

    IHC image of ASS1 staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins.  The section was then incubated with ab209018, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • HRP Anti-ASS1 antibody [EPR12398] (ab209018)
    HRP Anti-ASS1 antibody [EPR12398] (ab209018)

References (0)

Publishing research using ab209018? Please let us know so that we can cite the reference in this datasheet.

ab209018 has not yet been referenced specifically in any publications.

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