Recombinant HRP Anti-ATAD2 antibody [EPR12730] (ab201743)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12730] to ATAD2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-ATAD2 antibody [EPR12730]
See all ATAD2 primary antibodies -
Description
HRP Rabbit monoclonal [EPR12730] to ATAD2 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Saos-2 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12730 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 159 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 159 kDa). |
Target
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Function
May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells. -
Tissue specificity
Highly expressed in estrogen receptor positive breast tumors and in osteosarcoma tumors. -
Sequence similarities
Belongs to the AAA ATPase family.
Contains 1 bromo domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29028 Human
- Omim: 611941 Human
- SwissProt: Q6PL18 Human
- Unigene: 370834 Human
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Alternative names
- AAA nuclear coregulator cancer-associated protein antibody
- AAA nuclear coregulator, cancer-associated antibody
- ANCCA antibody
see all
Images
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All lanes : HRP Anti-ATAD2 antibody [EPR12730] (ab201743)
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ATAD2 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 159 kDa
Exposure time: 20 minutesab201743 was shown to recognize ATAD2 in wild-type HAP1 cells as signal was lost at the expected MW in ATAD2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ATAD2 knockout samples were subjected to SDS-PAGE. ab201743 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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HRP Anti-ATAD2 antibody [EPR12730] (ab201743) at 1/5000 dilution + Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201743 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab201743 has not yet been referenced specifically in any publications.