HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197905)
Key features and details
- HRP Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker
See all ATPB primary antibodies -
Description
HRP Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Caenorhabditis elegans, Monkey -
Immunogen
The details of the immunogen for this antibody are not available.
-
Positive control
- WB: Human heart mitochondrial lysate. HeLa and HepG2 whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
3D5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
|
Notes |
---|
WB
1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
-
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
Sequence similarities
Belongs to the ATPase alpha/beta chains family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
-
Database links
- Entrez Gene: 327675 Cow
- Entrez Gene: 506 Human
- Entrez Gene: 11947 Mouse
- Entrez Gene: 171374 Rat
- GenBank: BC016512 Human
- Omim: 102910 Human
- SwissProt: P00829 Cow
- SwissProt: P06576 Human
see all -
Alternative names
- ATP 5B antibody
- ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
- ATP synthase subunit beta mitochondrial antibody
see all
Images
-
All lanes : HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197905) at 1/5000 dilution
Lane 1 : Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Lane 2 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197905 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab197905 has not yet been referenced specifically in any publications.