Recombinant HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)
![Western blot - HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)](https://www.abcam.com/ps/products/209/ab209039/Images/ab209039-305102-ab209039HRPKOWBJRIZ.jpg "Western blot - HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17509] to Bcl-2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Bcl-2 antibody [EPR17509]
See all Bcl-2 primary antibodies -
Description
HRP Rabbit monoclonal [EPR17509] to Bcl-2 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Bcl-2 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P10415 -
Positive control
- WB: THP1 whole cell and Human Tonsil, Human Thymus and Mouse Spleen tissue lysates. IHC-P: normal human spleen tissue sections
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17509 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab209039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). -
Tissue specificity
Expressed in a variety of tissues. -
Involvement in disease
A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. -
Post-translational
modificationsPhosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. -
Cellular localization
Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 596 Human
- Entrez Gene: 12043 Mouse
- Omim: 151430 Human
- SwissProt: P10415 Human
- SwissProt: P10417 Mouse
- Unigene: 150749 Human
- Unigene: 257460 Mouse
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Alternative names
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
see all
Images
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Western blot - HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)All lanes : HRP Anti-Bcl-2 antibody [EPR17509] (ab209039) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : BCL2 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Exposure time: 20 minutesab209039 was shown to specifically react with Bcl-2 in wild-type HAP1 cells as signal was lost in BCL2 knockout cells. Wile-type and BCL2 knockout samples were subjected to SDS-PAGE. Ab209039 was incubated overnight at 4°C at 1/5000 dilution. Blots were developed with ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)](https://www.abcam.com/ps/products/209/ab209039/Images/ab209039-280881-IHCAP00255621.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)IHC image of Bcl-2 staining in a section of formalin-fixed paraffin-embedded normal human spleen*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab209039, 1/250 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)](https://www.abcam.com/ps/products/209/ab209039/Images/ab209039-270876-WBab2090391.jpg)
Western blot - HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)All lanes : HRP Anti-Bcl-2 antibody [EPR17509] (ab209039) at 1/5000 dilution
Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Tonsil (Human) Tissue Lysate - adult normal tissue
Lane 3 : Thymus (Human) Tissue Lysate - adult normal tissue
Lane 4 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209039 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)](https://www.abcam.com/ps/products/209/ab209039/Images/ab209039-4-benefits-of-recombinant-antibodies.png)
HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)
References (0)
ab209039 has not yet been referenced specifically in any publications.
