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    hrp-beta-actin-antibody-ba3r-ab173838.pdf

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Isotype/Loading Controls Loading Controls Beta Actin
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HRP Anti-beta Actin antibody [BA3R] (ab173838)

  • Datasheet
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Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)

    Key features and details

    • HRP Mouse monoclonal [BA3R] to beta Actin
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Rabbit, Chicken, Human
    • Conjugation: HRP
    • Isotype: IgG2b

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    Overview

    • Product name

      HRP Anti-beta Actin antibody [BA3R]
      See all beta Actin primary antibodies
    • Description

      HRP Mouse monoclonal [BA3R] to beta Actin
    • Host species

      Mouse
    • Conjugation

      HRP
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Rabbit, Chicken, Human
    • Immunogen

      Synthetic peptide corresponding to Human beta Actin (N terminal).
      Database link: P60709

    • Positive control

      • HeLa, 293T, U2OS, COS7, NIH 3T3 and NRK cell lysates.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Concentration information loading...
    • Clonality

      Monoclonal
    • Clone number

      BA3R
    • Isotype

      IgG2b
    • Research areas

      • Isotype/Loading Controls
      • Loading Controls
      • Beta Actin
      • Tags & Cell Markers
      • Subcellular Markers
      • Cytoskeleton
      • Actin
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin

    Associated products

    • Immunizing Peptide (Blocking)

      • Recombinant Human beta Actin protein (His tag) (ab240844)
    • Recombinant Protein

      • Recombinant Human beta Actin protein (His tag) (ab240844)
    • Related Products

      • HeLa whole cell lysate (ab150035)
      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)
      • HeLa whole cell lysate (ab29545)
      • NIH 3T3 whole cell lysate (ab7179)

    Applications

    Our Abpromise guarantee covers the use of ab173838 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/2000. Predicted molecular weight: 42 kDa.Can be blocked with Recombinant Human beta Actin protein (His tag) (ab240844).

    Target

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease

      Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
    • Sequence similarities

      Belongs to the actin family.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Target information above from: UniProt accession P60709 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 396526 Chicken
      • Entrez Gene: 60 Human
      • Entrez Gene: 11461 Mouse
      • Entrez Gene: 100009272 Rabbit
      • Entrez Gene: 81822 Rat
      • Omim: 102630 Human
      • SwissProt: P60706 Chicken
      • SwissProt: P60709 Human
      • SwissProt: P60710 Mouse
      • SwissProt: P29751 Rabbit
      • SwissProt: P60711 Rat
      • Unigene: 520640 Human
      • Unigene: 708120 Human
      • Unigene: 727576 Human
      • Unigene: 328431 Mouse
      • Unigene: 391967 Mouse
      • Unigene: 94978 Rat
      see all
    • Alternative names

      • A26C1A antibody
      • A26C1B antibody
      • ACTB antibody
      • ACTB_HUMAN antibody
      • Actin beta antibody
      • Actin cytoplasmic 1 antibody
      • Actin, cytoplasmic 1, N-terminally processed antibody
      • Actx antibody
      • b actin antibody
      • b-actin antibody
      • Beta cytoskeletal actin antibody
      • Beta-actin antibody
      • BRWS1 antibody
      • E430023M04Rik antibody
      • Melanoma X actin antibody
      • MGC128179 antibody
      • PS1TP5 binding protein 1 antibody
      • PS1TP5BP1 antibody
      see all

    Images

    • Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)
      Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)
      All lanes : HRP Anti-beta Actin antibody [BA3R] (ab173838) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : U2OS cell lysate
      Lane 4 : COS7 cell lysate
      Lane 5 : NIH 3T3 cell lysate
      Lane 6 : NRK cell lysate

      Lysates/proteins at 30 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 42 kDa



      4-20% Tris-HCl polyacrylamide gel.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (5)

    Publishing research using ab173838? Please let us know so that we can cite the reference in this datasheet.

    ab173838 has been referenced in 5 publications.

    • Pan Y  et al. Bromodomain PHD-finger transcription factor promotes glioma progression and indicates poor prognosis. Oncol Rep 41:246-256 (2019). PubMed: 30542695
    • Wang T  et al. Effects of leukemia inhibitory factor receptor on the adipogenic differentiation of human bone marrow mesenchymal stem cells. Mol Med Rep 19:4719-4726 (2019). PubMed: 31059010
    • Canzoneri R  et al. Alternative splicing variant of RHBDD2 is associated with cell stress response and breast cancer progression. Oncol Rep 40:909-915 (2018). PubMed: 29901166
    • Li X  et al. miR-377-3p regulates adipogenic differentiation of human bone marrow mesenchymal stem cells by regulating LIFR. Mol Cell Biochem N/A:N/A (2018). PubMed: 29959592
    • Windrem MS  et al. Human iPSC Glial Mouse Chimeras Reveal Glial Contributions to Schizophrenia. Cell Stem Cell 21:195-208.e6 (2017). PubMed: 28736215

    Customer reviews and Q&As

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