HRP Anti-beta Actin antibody [BA3R] (ab173838)
![Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)](https://www.abcam.com/ps/products/173/ab173838/Images/ab173838-197657-anti-beta-actin-antibody-ba3r-hrp-western-blot.jpg "Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)")
Key features and details
- HRP Mouse monoclonal [BA3R] to beta Actin
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-beta Actin antibody [BA3R]
See all beta Actin primary antibodies -
Description
HRP Mouse monoclonal [BA3R] to beta Actin -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Chicken, Human -
Immunogen
Synthetic peptide corresponding to Human beta Actin (N terminal).
Database link: P60709 -
Positive control
- HeLa, 293T, U2OS, COS7, NIH 3T3 and NRK cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
BA3R -
Isotype
IgG2b -
Research areas
Associated products
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Immunizing Peptide (Blocking)
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Recombinant Protein
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Related Products
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Applications
Our Abpromise guarantee covers the use of ab173838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 42 kDa.Can be blocked with Recombinant Human beta Actin protein (His tag) (ab240844). |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60709 Human
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)All lanes : HRP Anti-beta Actin antibody [BA3R] (ab173838) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U2OS cell lysate
Lane 4 : COS7 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lane 6 : NRK cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa4-20% Tris-HCl polyacrylamide gel.
Datasheets and documents
References (5)
ab173838 has been referenced in 5 publications.
- Pan Y et al. Bromodomain PHD-finger transcription factor promotes glioma progression and indicates poor prognosis. Oncol Rep 41:246-256 (2019). PubMed: 30542695
- Wang T et al. Effects of leukemia inhibitory factor receptor on the adipogenic differentiation of human bone marrow mesenchymal stem cells. Mol Med Rep 19:4719-4726 (2019). PubMed: 31059010
- Canzoneri R et al. Alternative splicing variant of RHBDD2 is associated with cell stress response and breast cancer progression. Oncol Rep 40:909-915 (2018). PubMed: 29901166
- Li X et al. miR-377-3p regulates adipogenic differentiation of human bone marrow mesenchymal stem cells by regulating LIFR. Mol Cell Biochem N/A:N/A (2018). PubMed: 29959592
- Windrem MS et al. Human iPSC Glial Mouse Chimeras Reveal Glial Contributions to Schizophrenia. Cell Stem Cell 21:195-208.e6 (2017). PubMed: 28736215
