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    hrp-beta-actin-antibody-mabcam-8224-loading-control-ab197277.pdf

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Isotype/Loading Controls Loading Controls Beta Actin
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HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277)

  • Datasheet
  • SDS
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Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277)

    Key features and details

    • HRP Mouse monoclonal [mAbcam 8224] to beta Actin - Loading Control
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Conjugation: HRP
    • Isotype: IgG3

    Conjugates logo Related conjugates and formulations

    Carrier Free Unconjugated

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    Overview

    • Product name

      HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control
      See all beta Actin primary antibodies
    • Description

      HRP Mouse monoclonal [mAbcam 8224] to beta Actin - Loading Control
    • Host species

      Mouse
    • Conjugation

      HRP
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
      Predicted to work with: Cow, Drosophila melanogaster, Schizosaccharomyces pombe, Chinese hamster
    • Immunogen

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
      (Peptide available as ab13772)

    • Positive control

      • WB: HEK293, NIH3T3 and PC12 whole cell lysates. Human skeletal muscle tissue lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Proclin 300 Solution
      Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Monoclonal
    • Clone number

      mAbcam 8224
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG3
    • Light chain type

      kappa
    • Research areas

      • Isotype/Loading Controls
      • Loading Controls
      • Beta Actin
      • Tags & Cell Markers
      • Subcellular Markers
      • Cytoskeleton
      • Actin
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin

    Associated products

    • Alternative Versions

      • Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)
      • Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
    • Immunizing Peptide (Blocking)

      • Human beta Actin peptide (ab13772)
    • Recombinant Protein

      • Recombinant Human beta Actin protein (His tag) (ab240844)
    • Related Products

      • ECL Substrate Kit (High Sensitivity) (ab133406)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab197277 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772).

    This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5% BSA. Incubate antibody in TBST for one hour or more.

    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772).

    This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5% BSA. Incubate antibody in TBST for one hour or more.

    Target

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease

      Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
    • Sequence similarities

      Belongs to the actin family.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Target information above from: UniProt accession P60709 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 280979 Cow
      • Entrez Gene: 60 Human
      • Entrez Gene: 11461 Mouse
      • Entrez Gene: 81822 Rat
      • Omim: 102630 Human
      • SwissProt: P48975 Chinese hamster
      • SwissProt: P60712 Cow
      • SwissProt: P60709 Human
      • SwissProt: P60710 Mouse
      • SwissProt: P60711 Rat
      • Unigene: 520640 Human
      • Unigene: 708120 Human
      • Unigene: 727576 Human
      • Unigene: 328431 Mouse
      • Unigene: 391967 Mouse
      • Unigene: 94978 Rat
      see all
    • Alternative names

      • A26C1A antibody
      • A26C1B antibody
      • ACTB antibody
      • ACTB_HUMAN antibody
      • Actin beta antibody
      • Actin cytoplasmic 1 antibody
      • Actin, cytoplasmic 1, N-terminally processed antibody
      • Actx antibody
      • b actin antibody
      • b-actin antibody
      • Beta cytoskeletal actin antibody
      • Beta-actin antibody
      • BRWS1 antibody
      • E430023M04Rik antibody
      • Melanoma X actin antibody
      • MGC128179 antibody
      • PS1TP5 binding protein 1 antibody
      • PS1TP5BP1 antibody
      see all

    Images

    • Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277)
      Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277)
      All lanes : HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277) at 1/5000 dilution

      Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
      Lane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa


      Exposure time: 10 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197277 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (8)

    Publishing research using ab197277? Please let us know so that we can cite the reference in this datasheet.

    ab197277 has been referenced in 8 publications.

    • Ku MW  et al. Intranasal vaccination with a lentiviral vector protects against SARS-CoV-2 in preclinical animal models. Cell Host Microbe 29:236-249.e6 (2021). PubMed: 33357418
    • Yi Z  et al. Comparative exome sequencing reveals novel candidate genes for retinitis pigmentosa. EBioMedicine 56:102792 (2020). PubMed: 32454406
    • Schröder C  et al. Late-Onset Antibody Deficiency Due to Monoallelic Alterations in NFKB1. Front Immunol 10:2618 (2019). PubMed: 31803180
    • Colombo M  et al. Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance. Haematologica N/A:N/A (2019). PubMed: 31582544
    • Zgheib R  et al. Folate can promote the methionine-dependent reprogramming of glioblastoma cells towards pluripotency. Cell Death Dis 10:596 (2019). PubMed: 31395852
    • Karbyshev MS  et al. Development of Novel Monoclonal Antibodies for Evaluation of Transmembrane Prostate Androgen-Induced Protein 1 (TMEPAI) Expression Patterns in Gastric Cancer. Pathol Oncol Res 24:427-438 (2018). PubMed: 28584893
    • Zafar MK  et al. A Small-Molecule Inhibitor of Human DNA Polymerase ? Potentiates the Effects of Cisplatin in Tumor Cells. Biochemistry 57:1262-1273 (2018). PubMed: 29345908
    • Wang J & Sun G FOXO1-MALAT1-miR-26a-5p Feedback Loop Mediates Proliferation and Migration in Osteosarcoma Cells. Oncol Res 25:1517-1527 (2017). PubMed: 28160461

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    Western blot abreview for Anti-beta Actin antibody [mAbcam 8224] - Loading Control (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African green monkey Cell lysate - whole cell (COS-7)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    10 µg
    Specification
    COS-7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 11 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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