HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.com/ps/products/20/ab20272/Images/ab20272-29577-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg "Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)")
Key features and details
- HRP Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Drosophila melanogaster, African green monkey, Chinese hamster
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control
See all beta Actin primary antibodies -
Description
HRP Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Drosophila melanogaster, African green monkey, Chinese hamster
Predicted to work with: Sheep, Horse, Guinea pig
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Immunogen
Synthetic peptide corresponding to Human beta Actin aa 1-100 conjugated to keyhole limpet haemocyanin (Sulfosuccinimidyl 4-N-maleimidomethyl-cyclohexane-1-carboxylate (Sulfo-SMCC)).
(Peptide available asab13772) -
Positive control
- HeLa whole cell lysate, 3T3 whole cell lysate, Rat brain lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam 8226 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092)
- Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576)
- Anti-beta Actin antibody [mAbcam 8226] - BSA and Azide free (ab264083)
- Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
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Immunizing Peptide (Blocking)
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Recombinant Protein
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Related Products
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- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
Our Abpromise guarantee covers the use of ab20272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 100033878 Horse
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
- Entrez Gene: 100009272 Rabbit
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : human testis lysate
Lane 2 : human brain lysate
Lane 3 : human tonsil lysate
Lane 4 : mouse brain lysate
Lane 5 : mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Exposure time: 2 minutes -
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.com/ps/products/20/ab20272/Images/ab20272-29576-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : 3T3 whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds -
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.com/ps/products/20/ab20272/Images/ab20272-29575-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)Lanes 1-4 : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% BSA)
Lanes 5-8 : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% MILK)
Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 & 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lanes 3 & 7 :NIH 3T3 whole cell lysate (ab7179)
Lanes 4 & 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minute
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
References (179)
ab20272 has been referenced in 179 publications.
- Cheung EC et al. Dynamic ROS Control by TIGAR Regulates the Initiation and Progression of Pancreatic Cancer. Cancer Cell 37:168-182.e4 (2020). PubMed: 31983610
- Nyati S et al. TGFBR2 mediated phosphorylation of BUB1 at Ser-318 is required for transforming growth factor-ß signaling. Neoplasia 22:163-178 (2020). WB . PubMed: 32143140
- Xu G et al. LBX2-AS1 up-regulated by NFIC boosts cell proliferation, migration and invasion in gastric cancer through targeting miR-491-5p/ZNF703. Cancer Cell Int 20:136 (2020). PubMed: 32351330
- Chia JSM et al. Zerumbone Modulates a2A-Adrenergic, TRPV1, and NMDA NR2B Receptors Plasticity in CCI-Induced Neuropathic Pain In Vivo and LPS-Induced SH-SY5Y Neuroblastoma In Vitro Models. Front Pharmacol 11:92 (2020). PubMed: 32194397
- Nakazawa H et al. Impaired social discrimination behavior despite normal social approach by kallikrein-related peptidase 8 knockout mouse. Neurobiol Learn Mem 162:47-58 (2019). PubMed: 31103466
