Application
Western blot
Sample
Human Cell lysate - whole cell (Breast epithelial)
Loading amount
25 µg
Specification
Breast epithelial
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/15000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% milk
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~40 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Nov 10 2009