Application
Western blot
Sample
Rat Cell lysate - whole cell (Neruo cell)
Gel Running Conditions
Reduced Denaturing (12 %)
Loading amount
20 µg
Specification
Neruo cell
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 42 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Ji Yeon Lee
Verified customer
Submitted Jan 28 2019