Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293)
Loading amount
50000 cells
Specification
HEK-293
Blocking step
Western Breeze Kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: Kit
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Secondary antibody
None used
Detection
Detection method
Western Breeze
Exposure
5 second(s)
Bands
Specific: 42 kDa
Additional data
Additional Notes
Excellent antibody - I tried it at 1/2000 dilution but actually 1/5000 would probably be even cleaner.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alan Hendrick
Verified customer
Submitted Apr 17 2007