Recombinant HRP Anti-beta Synuclein antibody [EP1537Y] (ab215783)
![Western blot - HRP Anti-beta Synuclein antibody [EP1537Y] (ab215783)](https://www.abcam.com/ps/products/215/ab215783/Images/ab215783-284007-WBab2157831.jpg "Western blot - HRP Anti-beta Synuclein antibody [EP1537Y] (ab215783)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1537Y] to beta Synuclein
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-beta Synuclein antibody [EP1537Y]
See all beta Synuclein primary antibodies -
Description
HRP Rabbit monoclonal [EP1537Y] to beta Synuclein -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human beta Synuclein aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q16143 -
Positive control
- Mouse brain, Human brain
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1537Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab215783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa). |
Target
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Function
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease. -
Tissue specificity
Expressed predominantly in brain; concentrated in presynaptic nerve terminals. -
Sequence similarities
Belongs to the synuclein family. -
Post-translational
modificationsPhosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6620 Human
- Entrez Gene: 104069 Mouse
- Omim: 602569 Human
- SwissProt: Q16143 Human
- SwissProt: Q91ZZ3 Mouse
- Unigene: 90297 Human
- Unigene: 200843 Mouse
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Alternative names
- 14 kDa brain-specific protein antibody
- Beta-synuclein antibody
- Phosphoneuroprotein 14 antibody
see all
Images
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Western blot - HRP Anti-beta Synuclein antibody [EP1537Y] (ab215783)All lanes : HRP Anti-beta Synuclein antibody [EP1537Y] (ab215783) at 1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215783 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-beta Synuclein antibody [EP1537Y] (ab215783)](https://www.abcam.com/ps/products/215/ab215783/Images/ab215783-2-benefits-of-recombinant-antibodies.png)
HRP Anti-beta Synuclein antibody [EP1537Y] (ab215783)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab215783 has not yet been referenced specifically in any publications.
